interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (hcl). the aim of the present study is to apply yeast strain for secreting human ifnα2b fol...

introduction: follicle stimulating hormone (fsh) is one of the pituitary glycoproteines that it consists of two subunits alpha and beta. the beta subunit is responsible for the biological activity of fsh. the aim of present study was isolation of the β subunit coding sequence containing its signal sequence from human genome and then cloning of the isolated sequence in ppic9 shuttle vector under...

background: thalassemia syndromes are the most prevalent single gene disorders in iran. this study aimed to evaluate the effect of different types of beta-globin gene mutations, co-inheritance of alpha-globin gene mutations and/or xmn1 snp on disease phenotype in a large cohort of iranian patients. subjects and methods: in total, 433 patients were clinically classified into β-thalassemia major ...

Abstract Chronic alcohol consumption is associated with significant mortality and morbidity in people affected by Mycobacterium tuberculosis (Mtb) infection. Our Lab previously reported that diet increased young mice infected Mtb an Interferon-α (IFN-α) dependent manner. We also found CD11b+Ly6g+ cells are major source for IFN-α. In the current study, using single cell RNA sequencing, we furthe...

background: aberrant methylation of cytosine-guanine dinucleotide islands leads to inactivation of tumor suppressor genes in breast cancer. tumor suppressor genes are unmethylated in normal tissue and often become hypermethylated during tumor formation, leading to gene silencing. we investigated the association between e-cadherin ( cdh1) and estrogen receptor-α (esrα) gene promoter methylation ...

This is a report of couple with abnormal hematological indices who were investigated for α & β-thalassemia mutations. Based on CBC and capillary hemoglobin electrophoresis results, the male female subjects β α-thalassemia carriers, respectively. Multiplex-Gap-PCR Sanger sequencing techniques used identification mutations β-globin genes. The DNA test showed presence c.315 + 1 G > A mutati...

Objective(s): Transforming growth factor alpha (TGF-α) has been shown to modulate the gastric acid secretion. Therefore, the aim of the present study was to investigate the effect of sodium hydrosulfide (NaHS) on TGF-α expression in gastric mucosa in rats. Materials and Methods: Eighteen rats were randomly divided into 3 groups (6 per group). To determine the effect of NaHS on gene and protein ...

The current study aimed to elucidate the potential involvement of mitochondria-endoplasmic reticulum contact genes in pathogenesis periodontal disease by monitoring levels associated including Mitofusion 1 (MFN1) and MFN2, inositol 1,4,5-trisphosphate receptor (IP3R), chaperone glucose-regulated protein 75 (GRP75), sigma non-opioid intracellular (SIGMAR1) phosphate tensin homolog induced putati...

introduction: helicobacter pylori (h. pylori), is a bacterium responsible for upper gastrointestinal tract diseases. the 16s rrna is a common h. pylori gene which are usually preferred for diagnosis purpose. the aim of this study was to determine the prevalence and abundance of 16s rrna in fecal samples and also evaluate correlation between the level of 16s rrna and activities of the cytokines,...

background inflammatory bowel disease (ibd) is a chronic, relapsing, inflammatory disorder of the gastrointestinal tract that includes two entities, crohn`s disease (cd) and ulcerative colitis (uc). as with other complex diseases, both genetic susceptibility and environmental factors play role in the pathogenesis of these diseases. the tumor necrosis factor α (tnf-α) gene is located in the ibd3...