α-Amylase from Trichoderma harzianum was covalently immobilized on activated wool by cyanuric chloride. Immobilized α-amylase exhibited 75% of its initial activity after 10 runs. The soluble and immobilized α-amylases exhibited maximum activity at pH values 6.0 and 6.5, respectively. The immobilized enzyme was more thermally stable than the soluble one. Various substrates were hydrolyzed by imm...

It is believed that the inhibition of carbohydrate hydrolyzing enzymes including α-amylase and α-glucosidase is one of the therapeutic approaches to decrease the postprandial glucose level after a meal, especially in the people with type 2 diabetes. Medicinal plants and their extracts are one of the main sources to find new inhibitors to the enzymes. In our study four flavonoids, namely luteoli...

Despite the presence of a family of defense proteins, Phaseolus vulgaris can be attacked by bruchid insects resulting in serious damage to stored grains. The two distinct active forms of α-amylase inhibitors, α-AI1 and α-AI2, in P. vulgaris show different specificity toward α-amylases. Zabrotes subfasciatus α-amylase is inhibited by α-AI2 but not by α-AI1. In contrast, porcine α-amylase is inhi...

Background: The purpose of this study was to evaluate α-amylase inhibitory effects hydroethanolic extracts bark from Daniella oliveri, Sclerocarya birrea, Maranthes polyandra, and Pteleopsis suberosa fight type-II diabetes. Methods: Compound extractions were performed by hydroethanol maceration followed liquid-liquid fractionation with solvents. TLC profiling carried out different fractions. pl...

Midgut α-amylase is an important digestive enzyme involved in larval energy metabolism and carbohydrate assimilation. In this article, the properties of midgut α-amylase from the Oriental armyworm, Mythimna separata (Lepidoptera: Noctuidae), larvae were characterized, and its in vitro responses to chemical inhibitors were also determined. The kinetic parameters Km and Vmax of midgut α-amylase w...

Polyphenolic compounds are known to be useful in formulating of nutritional or medicinal supplements for the treatment of several diseases. An important activity of polyphenols is the inhibition of digestive enzymes, especially carbohydrate-hydrolyzing enzymes such as α-amylase and α-glucosidase. Inhibitors of these enzymes are able to retard carbohydrate digestion, thus causing a reduction in ...

Alzheimer's disease (AD) and diabetes mellitus (DM) are related to abnormal changes in enzyme activity. While acetylcholinesterase (AChE) butyrylcholinesterase (BChE) the primary targets treatment of (AD), α-glucosidase (α-Gly) α-amylase (α-Amy) enzymes known for (DM). Here, benzenesulfonamide-based thiourea thiazolidinone derivatives such as AChE, BChE, α-Gly, α-Amy inhibitors were reported. T...

Diabetes mellitus is a multifactorial metabolic disease characterized by post-prandial hyperglycemia (PPHG). α-amylase and α-glucosidase inhibitors aim to explore novel therapeutic agents. Herein we report the promises of Dioscorea bulbifera and its bioactive principle, diosgenin as novel α-amylase and α-glucosidase inhibitor. Among petroleum ether, ethyl acetate, methanol and 70% ethanol (v/v)...

The side effects associated with the usage of synthetic antidiabetic drugs make it imperative to search for alternative drugs from medicinal plants. Therefore, this study was aimed at evaluating the α-amylase and α-glucosidase inhibitory potential of Calotropis procera leaf, as well as its possible mode of inhibiting these enzymes. Acetone, aqueous and ethanolic extracts of C. procera leaf was ...

This study aimed to evaluate in vitro free radical scavenging activity and anti-diabetic properties of extracts from the sprout Borassus fabellifier. Antioxidant was performed using DPPH, ABTS, FRAP, SO, NO inhibition methods. The enzymes (α-amylase α- Glucosidase inhibitory activities were investigated. Moreover, glucose adsorption diffusion also studied confirm potential effect B. fabellifier...