AIM To access beta-endorphin levels in serum as well as seminal plasma in different infertile male groups. METHODS Beta-endorphin was estimated in the serum and seminal plasma by enzyme-linked immunosorbent assay (ELISA) method in 80 infertile men equally divided into four groups: non-obstructive azoospermia (NOA), obstructive azoospermia (OA), congenital bilateral absent vas deferens (CBVAD)...

The paraventricular hypothalamus regulates autonomic nerve outflow and is innervated with /3-endorphin-immunoreactlve nerve terminals. This study examined the effects of /3-endorphin microinjected into the paraventricular hypothalamus on blood pressure, heart rate, and plasma catecholamine and glucose concentrations in conscious, unrestrained spontaneously hypertensive rats (SHR) and Wistar-Kyo...

a-Neo-endorphin anddynorphin immunoreactivities in rat brain were visualized by double antibody immunofluorescence of frozen sections. Antibodies were used that were specific for their respective antigens. The pattern of neuronal immunostaining produced by a-neo-endorphin and dynorphin antisera in adjacent serial sections was completely superimposible. No areas were found in which a-neo-endorph...

This cross-sectional study aimed to assess perceptions of healthy food and motivation for health behaviour (MHB) in different Mediterranean countries. Multiple linear regression was used identify the association between perception MHB The highest observed Portugal (median 38.0; IQR 7.0) lowest Greece 34.0; 8.0). Compared Portuguese respondents, respondents from Croatia (ß=0.35; p < 0.001), Egyp...

An effect of metrizamide, a contrast medium, on results of beta-endorphin radioimmunoassay was examined. We found that 1, 5, and 10 microL of the medium added to 100 microL of standard containing 0 to 500 pg of beta-endorphin shifted the standard curve to the left in proportion to the metrizamide concentration. Three other contrast media showed a similar effect at low concentrations of beta-end...

The present study was performed to investigate the effect of 0-endorphin on macrophage colony-stimulating factor (MCSF)-induced differentiation of macrophages from bone marrow cells in a semisolid culture system. 0-endorphin increased the number of macrophage colonies when bone marrow cells were cultured in the presence of M-CSF plus lipopolysaccharide (LPS). This was not the case with LPSunres...

The paraventricular hypothalamus regulates autonomic nerve outflow and is innervated with beta-endorphin-immunoreactive nerve terminals. This study examined the effects of beta-endorphin microinjected into the paraventricular hypothalamus on blood pressure, heart rate, and plasma catecholamine and glucose concentrations in conscious, unrestrained spontaneously hypertensive rats (SHR) and Wistar...

Antisera to the naloxone-insensitive receptor for beta-endorphin expressed on the U937 cell line were generated by using the complementary peptide strategy. A nanopeptide complementary to a C-terminal fragment of human beta-endorphin was synthesized as predicted by reading the beta-endorphin antisense mRNA 3' to 5'. By using enzyme-linked immunosorbent assay, rabbit antisera specific for the pe...