COPII vesicles transport proteins destined for secretion from the ER to the Golgi apparatus. A recent study has shown that, in budding yeast, the formation of COPII vesicles requires Yip1p, an effector protein of a Rab GTPase.

In yeast and animals, cyclins have been demonstrated to be important regulators of cell cycle progression. In recent years, a large number of A-, B-, and D-class cyclins have been isolated from a variety of plant species. One class of cyclins, the D-class cyclins, is important for progression through G1 phase of the cell cycle. In Arabidopsis, four D-class cyclins have been isolated and charact...

BACKGROUND Knowledge of the causative organism(s) in bacterial rhinosinusitis has become the cornerstone of adequate medical and surgical management. Little uniformity and data exist for the best method of obtaining sinus cultures. Most otolaryngologists tend to use the nasal swab for obtaining transnasal middle meatal cultures. A prospective study was carried out to compare the effectiveness o...

Abstract Plant peroxidases are important for several processes, such as defense against pathogens, and auxin metabolism. In this study, we report the active production secretion of plant peroxidase mutated enzymes in bacterium Brevibacillus choshinensis first time world. We introduced mutations into prxA3a , an anionic gene hybrid aspen, Populus × kitakamiensis to substitute amino acid residues...

Tephritid fruit flies, including Ceratitis ditissima, often invade citrus orchards. These flies cause economic losses to farmers and can prevent from exporting their fruits foreign markets. To detect the presence of in orchards, traps are baited with synthetic lures, which expensive for smallholder farmers. Farmers developing or financially less-endowed countries have import such baits, raising...

O-glycosylation has been considered a limiting factor in protein secretion in filamentous fungi. Overexpression of the yeast DPM1 gene encoding dolichylphosphate mannose synthase (DPMS) in an Aspergillus nidulans mutant (BWB26A) deficient in O-glycosylation caused an increase in the number of secretory vesicles and changes in protein secretion. However, the secretory proteins, primarily O-manno...

In order to identify the function of deafness locus putative guanine nucleotide exchange factor (DelGEF), a protein homologous to the nucleotide exchange factor for the small GTPase Ran, a cDNA library was screened for interacting proteins using a yeast two-hybrid system. The human homologue of Sec5, a protein involved in vesicle transport and secretion, was identified as a binding partner. The...

BACKGROUND Extracellular vesicles in yeast cells are involved in the molecular traffic across the cell wall. In yeast pathogens, these vesicles have been implicated in the transport of proteins, lipids, polysaccharide and pigments to the extracellular space. Cellular pathways required for the biogenesis of yeast extracellular vesicles are largely unknown. METHODOLOGY/PRINCIPAL FINDINGS We cha...

conclusions we found a marked increase in the incidence of non-albicans species (48.2%) among neutropenic patients. only a few strains failed to produce extracellular enzymes. finally, in addition to fluconazole, caspofungin can be considered as the first line treatment against candida species among neutropenic patients. results a total of 110 yeast strains isolated from urine and oral cavities...