The identification of transformant bacterial colonies containing potential recombinant DNA clones can be performed using a wide variety of screening methods including colony hybridization and autoradiography, polymerase chain reaction (PCR) and restriction endonuclease analysis of individual plasmid DNA (1,5). While colony hybridization can efficiently screen large numbers of recombinant clones...

The organization of repetitive sequences within three cloned chromosomal segments from human fetal liver DNA containing the initiator methionine tRNA gene was studied. The procedure developed for this study involves the contact hybridization of an electrophoretically separated 5'-32P-labeled restriction endonuclease digest of the cloned segment with total human genomic DNA covalently bound to a...

Next-generation sequencing has still not reached its full potential due to the technical inability of effectively targeting desired genomic regions of interest. Once available, methods adressing this bottleneck will dramatically reduce cost and enable the efficient analysis of complex samples. Recently, a number of possible approaches for genomic-scale sequence enrichment have been reported usi...

Long noncoding RNAs (lncRNAs) have important regulatory roles and can function at the level of chromatin. To determine where lncRNAs bind to chromatin, we developed capture hybridization analysis of RNA targets (CHART), a hybridization-based technique that specifically enriches endogenous RNAs along with their targets from reversibly cross-linked chromatin extracts. CHART was used to enrich the...

A novel, voltage-gated sodium channel cDNA, designated NaCh6, has been isolated from the rat central and peripheral nervous systems. RNase protection assays showed that NaCh6 is highly expressed in the brain, and NaCh6 mRNA is as abundant or more abundant than the mRNAs for previously identified rat brain sodium channels. In situ hybridization demonstrated that a wide variety of neurons express...

Increasing the sensitivity in DNA microarray hybridization can significantly enhance the capability of microarray technology for a wide range of research and clinical diagnostic applications, especially for those with limited sample biomass. To address this issue, using reverse microemulsion method and surface chemistry, a novel class of homogenous, photostable, highly fluorescent streptavidin-...

The genes encoding the NADH dehydrogenase subunits of respiratory complex I have not been identified so far in the mitochondrial DNA (mtDNA) of yeasts. In the linear mtDNA of Candida parapsilosis, we found six new open reading frames whose sequences were unambiguously homologous to those of the genes known to code for NADH dehydrogenase subunit proteins of different organisms, i.e., ND1, ND2, N...

The possibility of occurrence of some rearrangements inside and or around coxII and atp6 genes and their relationship with male sterility in rice lines having wild abortive (WA)  cytoplasm was studied. Two sterile (IR58025A and IR62829A) and two maintainer lines (IR58025B and IR62829B) were used. Radioactive Southern blotting was employed to carry out the experiments. The hybridization of coxII...