Mitochondrial DNA replication is performed by a simple machinery, containing the TWINKLE DNA helicase, a single-stranded DNA-binding protein, and the mitochondrial DNA polymerase γ. In addition, mitochondrial RNA polymerase is required for primer formation at the origins of DNA replication. TWINKLE adopts a hexameric ring-shaped structure that must load on the closed circular mtDNA genome. In o...

Circular clamps are utilised by replicative polymerases to enhance processivity. The topological problem of loading a toroidal clamp onto DNA is overcome by ATP-dependent clamp loader complexes. Different organisms use related protein machines to load clamps, but the mechanisms by which they utilise ATP are surprisingly different. Using mutant clamp loaders that are deficient in either ATP bind...

jMonitor is a pure Java library and runtime utility for specifying event patterns and associating them with user provided event monitors that get called when the specified Java runtime events occur during the execution of legacy Java applications. jMonitor APIs define an event specification abstraction layer allowing programmers to design event patterns they would like to monitor during the run...

With the development of space science and technology, some countries have launched unmanned planetary exploration programs. Because planet surfaces are rough and formidable, planetary exploration rovers were used as a versatile and safe alternative to manned space missions to explore the planets. In recent years, many types of planetary rovers were developed including tracked type, legged type,...

A unique polygenic model of rat physical activity has been recently developed where rats were selected for the trait of low voluntary wheel running. We utilized this model to identify differences in soleus and plantaris muscles of sedentary low voluntary wheel running rats and physically active low voluntary wheel running rats exposed to moderate amounts of treadmill training. Three groups of 2...

Rail wheel squeal on curves in rail track is a source of potential noise annoyance to residents adjacent to rail lines. For one private rail freight operator, wheel squeal was causing repeated complaints from neighbours. Wheel squeal can be caused by the wheel-tyre to rail-top interaction and the wheel flange interaction with the gauge face of the rail (more like a screech). This paper describe...

Abstract: To improve the stability and reliability of the piezoelectric micro-pump, the cause of air block phenomenon is analyzed on the structure of wheel check valve. During the movement of the bubble in the micro-channel, pressure drop occurs, the main factor which influences the bubble going through is opening height of the wheel check valve. Five groups of wheel check valves with different...

This paper discuses sensor steering mechanism (SSM) for a laterally guided vehicle with a rear wheel steering mechanism. The authors have demonstrated the geometry of SSMs for front wheel steering type and reverse phase four-wheel steering type vehicles. SSMs allow stable lateral guiding performance for automated vehicles following a path with straight and curved portions created by a guideway....

Many patents have suggested that spinning the aircraft wheel before touchdown would lessen tyre wear as indicated by landing smoke and rubber deposites on the runway caused by skidding wheel at the point of impact. In this paper, the required torque to spin the aircraft wheel at approach speed has been calculated using ANSYS Workbench CFX, which is used to determine the wheel aerodynamic forces...