Vitamins are now available at reasonable prices, much is known about modes of action and there is a rich literature on dietary surveys. Forty years ago the classical deficiency diseases were rampant and Edward Mellanby used to say that the difficulty was ‘to get vitamins down the throats of those who need them’. Since that time normal diets have improved and welfare schemes have been adopted so...

Over a period of five years, 123 patients who had 124 limb amputations in King Fahd Hospital, Jeddah, Western Saudi Arabia were reviewed to find out the pattern of amputations, aetiological factors and difficulties in management. The male: female ratio was 2.8 : 1. The mean age was 52.5 years (range, 2·100 yrs). The lower limb was involved in 108 cases (87.8%) compared to only 16 upper limb amp...

MOTIVATION A critical task in high-throughput sequencing is aligning millions of short reads to a reference genome. Alignment is especially complicated for RNA sequencing (RNA-Seq) because of RNA splicing. A number of RNA-Seq algorithms are available, and claim to align reads with high accuracy and efficiency while detecting splice junctions. RNA-Seq data are discrete in nature; therefore, with...

According to Patrick Colm Hogan, in the US academic context, few people in literary theory or comparative literature have much familiarity with non-Western literary theories, and fewer still have research expertise in the field. While working on a project in non-Western literary theory, he was surprised to find that many of his friends and colleagues found it difficult to understand what non-We...

Objective(s):Antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. The aim of this study was to produce polyclonal antibody against a synthetic peptide derived from N-terminal region of β-actin protein to be used as a protein loading control in western ...

Background and Aims: Herpes simplex virus type 1 (HSV1) remains a potentially serious health problem world wide. All infected people, including asymptomatic ones, are potential sources for virus transmission. Virus envelope contains at least 13 glycoproteins, which glycoprotein D is the major target of immune responses. The aim of this study was development of a specific method that is a more...

Western blotting is a commonly used technique in biological research. A major problem with Western blotting is not the method itself, but the use of poor quality antibodies as well as the use of different experimental conditions that affect the linearity and sensitivity of the Western blot. Investigation of some conditions that are commonly used and often modified in Western blotting, as well a...

in this study 19 blv infected and 2 healthy lymph nodes were analysed by western blotting to detect viral p 24 antigen . western blotting was carried out by rabbit anti p24 serum and p24 monoclonal antibody. rabbit serum in western blotting could detect 45, 50, 54, 58 and less than 19 kd bands of proteins and monoclonal antibody could detect 30, 32, 39, 45, 50 and 53 kd bands. our results indic...