A time-resolved fluoroimmunoassay (TR-FIA) was established and validated that allows for the determination of plasma concentrations of insulin-like growth factor I (IGF-I) in three domestically cultured fishes: channel catfish (Ictalurus punctatus), hybrid striped bass (Morone chrysopsxM. saxatilis), and rainbow trout (Oncorhynchus mykiss). Sensitivity of the assay was 0.20 ng/ml. Intra- and in...

Two experiments were conducted to study the effects of feeding freqnency on growth, and of handling stress on growth and mortality, in intensive tank culture of the walking catfish Clunas fuscus. In the first experiment, fish were grown from an initial weight of 37 g for 34 d. A fixed ration of 3.0% body weight per day was divided into either 1, 2, or 3 feedings per day, and fish were either ha...

Channel catfish Ictalurus punctatus fingerlings were fed purified diets supplemented with iron at levels of 0, 20, 60, and 180 mgkg from iron sulfate (FeS) or 5, 10, 20, 60, and 180 mgkg from iron methionine (FeM) in triplicate tanks for 8 wk. Fish were then divided into two groups and subjected to different assays to measure disease resistance and individual immune functions. Representative fi...

A study was carried out to evaluate the probiotic activity of Bacillus subtilis G1 isolated from fermented pickles in growth performance and disease resistance of Hemibagrus nemurus fingerlings at Universiti Putra Malaysia. The probiotic was mixed in feed at doses of 0 (C, control), 3 x 109 (T1) 3 x 107 (T2) and 3 x 105 (T3) cfu g-1 and fed to the catfish fingerlings for nine weeks. Results sho...

Background The objective of the present study was to evaluate the growth performance and feed utilization of African catfish fed a commercial diet and Clarias gariepinus reared in the biofloc system enhanced with probiotic. Methods The treatment was the frequency of probiotic application into the cultured system, namely, 5-day interval, 10-day interval, and 15-day interval for 60 days of experi...