The yeast cwh36Delta mutant was identified in a screen for yeast mutants exhibiting a Vma(-) phenotype suggestive of loss of vacuolar proton-translocating ATPase (V-ATPase) activity. The mutation disrupts two genes, CWH36 and a recently identified open reading frame on the opposite strand, YCL005W-A. We demonstrate that disruption of YCL005W-A is entirely responsible for the Vma(-) growth pheno...

OBJECTIVE To investigate methods for improving the preoperative management of patients with pheochromocytoma. METHODS We collected 155 cases diagnosed as adrenal pheochromocytoma and with hypertension. During preoperative preparation, 85 patients had antihypertensive therapy by using the selective α1-blocker doxazosin controlled-release tablets; 70 patients was controlled by using the nonsele...

Inteins are coding sequences that are transcribed and translated with flanking sequences and then are excised by an autocatalytic process. There are two types of inteins in fungi, mini-inteins and full-length inteins, both of which present a splicing domain containing well-conserved amino acid sequences. Full-length inteins also present a homing endonuclease domain that makes the intein a mobil...

Functional electrical stimulation is a rehabilitation technique used to restore the motor muscular function by means of electrical stimulus commanded by a trigger signal under volitional control. In order to enhance the motor rehabilitation, a more convenient control signal may be provided by the same muscle that is being stimulated. For example, the tibialis anterior (TA) in the applications o...

The proton-translocating ATPase (H(+)-ATPase) found on the membrane of the yeast vacuole is the best characterized member of the V-type ATPase family. Biochemical and genetic screens have led to the identification of 14 genes, the majority designated VMA (for vacuolar membrane ATPase) encoding subunits of the enzyme complex. At least eight genes encode for proteins comprising the peripherally a...

Gypsum waste is from the remnants of making gypsum lists that cannot be recycled and reused. In this study, powder was used as a filler substitute (cement). Where cement contains high enough lime (CaO). The aims study are to determine how process mixtures Aspal Concrete–Wearing Course (AC–WC) by using (cement) results Marshall test, namely stability, VFWA, VMA, VIM, Flow, MQ (cement).. method i...

Penelitian ini berfokus untuk mengetahui bagaimana pengaruh penambahan Styrofoam sebagai bahan tambah Adiktif pada campuran AC-WC dengan menggunakan Batu Sungai Pattunuang Kabupaten Maros material, metode penelitian yang digunakan adalah serangkaian pengujian Marshall konvensional, Hasil variasi kadar 0%, 1%, 2%, 3%, 4% komposisi agregat kasar 36,90%, halus 50%30, filler 5,8%, aspal 5,5 % diper...