background: transient and stable transformation of host plants are the common techniques to produce transgenic plants. however, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. while, transient gene expression is a quick method to produce recombinant proteins in plants.  objective: the main goal of the present study was to eval...

stability study of biological products plays an important role for determination of product changes in maintenance period and ensuring of safety and efficacy of vaccines. in this research, accelerated and long-term stability study performed for six batches of full and reduced-dose cattle iriba strain brucellosis vaccine that manufactured by razi vaccine and serum research institute as a new vac...

background : bacterial infections in burn and wound patients are common and difficult to control. the aim of the current study was to evaluate the ability of full length oprf to elicit the production of protective igg in mice burn wound sepsis model against p. aeruginosa infection. methods : oprf protein was expressed and purified by ni-nta. the purified protein as used to immunize balb/c mice....

an oil adjuvant vaccine (oav) was prepared from a local strain of pasteurella multocida. strain 6:b was cultured and inactivated by formalin. bacterial pellet was prepared by centrifugation and subsequently adjuvanted by montannide oil isa-70. a dose of prepared vaccine containing 3ml (2mg dry weight/ml) was injected into five calves by im route. animals were bled before and at 24, 90, 150, and...

pseudomonas aeruginosa as an opportunistic pathogen is a significant cause of acute and chronic infections in patients with compromised defenses. this bacterium is motile via a single polar flagellum made of polymerized flagellin subunits differentiated into two major serotypes: a and b. flagellin plays an important role as a virulence factor in the adhesion, colonization and invasion of p. aer...

objective: animal studies show that vaccination with epitope-based peptides results in protective immunity. however, immunodominance should be regarded as a major challenge in this area. considering the advantages of epitopic-vaccines against hepatitis c virus (hcv) infection, herein, we compared the occurrence of immunodominance following mice immunization with three different hcv epitopic-pep...

the role of vi-capsular polysaccharide (vi-cps) in human immunity against infection caused by salmonella typhi is well known. the downstream process of purification generally causes depolymerization of vi-cps to a nonimmunogenic low molecular weight form. in the present study, a standard strain of sal. typhi ty6s was grown under submerge cultural conditions in a pilot-plant scale of 90 liter fe...

introduction: long term complications of hepatitis c virus (hcv) infection include fibrosis, cirrhosis, and hepatocellular carcinoma and although the disease is treatable with the newly introduced direct acting antivirals, but factors such as drug resistance, viral genotype and adverse effects can limit the effectiveness of therapy. therefore, development of effective, safe and affordable proph...

ontology is a requirement engineering product and the key to knowledge discovery. it includes the terminology to describe a set of facts, assumptions, and relations with which the detailed meanings of vocabularies among communities can be determined. this is a qualitative content analysis research. this study has made use of ontology for the first time to discover the knowledge of vaccine in ir...