A cis-acting enoyl reductase (ER) catalytic domain was isolated from a fungal highly reducing iterative polyketide synthase (HR-iPKS) for the first time and studied in vitro. The ER from the squalestatin tetraketide synthase forms a discrete dimeric protein in solution. The ER shows broad substrate selectivity, reducing enoyl species including both natural and unnatural substrates. Pantetheine-...

ÐThe need for readily biodegradable organic matter to support denitri®cation and enhanced biological phosphorus removal (EBPR) can be satis®ed using external or internal carbon sources. This paper examines the production and removal of volatile fatty acids (VFAs), mainly acetic acid, and other hydrolyzed products from sludge using hydrothermal treatment at subcritical (<3748C) and supercritical...

Laboratory column tests conducted to gain insight regarding the biological and chemical clogging mechanisms in a porous medium are presented. To seed the porous medium with landfill bacteria, a mixture of Keele Valley Landfill and synthetic leachate permeated through the column under anaerobic conditions for the first 9 days of operation. After this, 100% synthetic leachate was used. The synthe...

This study was conducted to determine if adding the acid-tolerant engineered bacterial strain M. elsdenii H6F32 to the rumen of sheep would reduce the risk of lactic acidosis. Lactic acidosis were induced by feed challenges in three groups of six sheep intraruminally dosed with wheat. In each group, sheep were not supplemented(C) or supplemented with the original bacterial strain M.elsdenii H6(...

The study appraised the effects of Carotino oil on in vitro rumen fermentation, gas production, metabolism and apparent biohydrogenation of oleic, linoleic and linolenic acids. Carotino oil was added to a basal diet (50% concentrate and 50% oil palm frond) at the rate of 0, 2, 4, 6 and 8% dry matter of the diet. Rumen inoculum was obtained from three fistulated Boer bucks and incubated with 200...

Current mechanistic anaerobic digestion (AD) models cannot accurately represent the underlying processes occurring during food waste (FW) AD. This work presents an update of Anaerobic Digestion Model no. 1 (ADM1) to provide accurate estimations free ammonia concentrations and related inhibition thresholds, model syntrophic acetate oxidation as acetate-consuming pathway. A modified Davies equati...

• The influence of different Household Food Wastes fractions on VFA was investigated. Fractions rich in proteins and starch led to the highest yield (12–15 g/L). cellulose, fibers, sugars showed low production (<2 Metagenomic analysis HFW VFAs conducted. bacterial community compositions profile correlated. This research investigated for first time single (proteins, lipids, starch, fibers sugars...

The aim of the study was to evaluate effect crude protein (CP) levels in concentrate and Sesbania grandiflora pod meal (SG) supplementation on feed intake, rumen fermentation, methane (CH4) mitigation Thai purebred beef cattle. Four cattle with 100 ± 5.0 kg body weight were used this study. A 2 × factorial experiment a 4 Latin square design conducted, which factor CP 14% 16% dry matter (DM) B s...

The wastewater sector paradigm is shifting from treatment to resource recovery. In addition, concerns regarding sustainability during the operation have increased. this sense, there a need break barriers (i.e., social, economic, technological, legal, etc.) for moving forward towards water recovery facilities and demonstration case studies can be very effective insightful. This paper presents ne...