Pheromone-responsive plasmids constitute a unique group of approximately 20 plasmids identified, as yet, only among enterococcal species. Several of their representatives, e.g. pAD1, pCF10, pPD1 and pAM373 have been extensively studied. These plasmids possess a sophisticated conjugation mechanism based on response to sex pheromones--small peptides produced by plasmid-free recipient cells. Detai...

Objective: An irreversible process begins when a systemic infection causes sepsis. Therefore, rapid identification of the agent bacteria in sepsis and its antibiotic resistance is crucially important. In this study, it was aimed to investigate efficiency genotype test.
 Methods: Blood culture bottles sent laboratory were examined with an automatic blood system (BACTEC, BD, USA). Growing id...

Recent cases of infections caused by glycopeptide-resistant enterococci (GRE) have highlighted the emergence of these organisms in the Republic of South Africa. During May 1998 we conducted a prevalence study in four hospitals in Johannesburg and obtained 184 rectal swabs from patients identified as being at high risk for GRE colonization. Twenty enterococcal isolates showing various glycopepti...

BACKGROUND Vancomycin-resistant enterococci pose an emerging health risk. The limitation in therapeutic options has resulted in the development of new drugs such as quinupristin/ dalfopristin and linezolid. AIM, SETTING AND DESIGN: This study investigated the species prevalence and antibacterial resistance among enterococci isolated in selected Tehran hospitals. MATERIALS AND METHODS Between ...

A mouse model of vancomycin-resistant Enterococcus faecium (VRE) intestinal colonization was used to study the effect of different subcutaneous antibiotics on persistence and density of VRE colonization. Gastric inoculation of a clinical VanB VRE isolate, in conjunction with oral vancomycin in drinking water (250 microgram/mL), resulted in high-level VRE colonization (mean, 9.5 log10 cfu/g) in ...

A mouse model of intestinal colonization with vancomycin-resistant enterococci (VRE) was used to study the effect of different beta-lactam antibiotics on establishment of VRE colonization. A clinical VanB VRE isolate, Enterococcus faecium C68 (102 or 104 cfu), was inoculated by gastric gavage in conjunction with subcutaneous administration of antibiotics. The MIC of ceftriaxone and ticarcillin ...

Results The colonisation rate was 78% (78/100). Ten grew non multi-resistant Grampositives MSSA (1) and Enterococcus species (9), 17 grew commensals. Non multiresistant Gram-negatives grew in 38 specimens: Pseudomonas species (13) and ‘coliforms’ (26). MROs were found on 25% of tourniquets, including 3 from MRO isolation rooms. An IMP-4 positive E. cloacae and an ESBL E. cloacae were isolated f...

We compared ChromID VRE and Brilliance VRE media for the detection of vancomycin-resistant enterococci (VRE). Using a panel of 28 enterococcal isolates, 10 vanA Enterococcus faecium and three vanA Enterococcus faecalis isolates grew as per manufacturers' instructions whilst growth of two vanC and eight vancomycin-susceptible enterococci was inhibited on both media. Important differences were no...