background and aims: certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. reverse transcription-polymerase chain reaction (rt-pcr) has been documented as an alternative assay for certificat...

conclusions the results of the present study showed that in comparison with previously published primer sets, p. aeruginosa infection can be diagnosed with higher sensitivity and specificity by the conventional pcr assay using the newly designed primers. it was also shown that the results of the pcr assay on clinical samples of severe infections became positive much earlier than the results of ...

plagiarism refers to “adopting someone else’s words, work or ideas and passing them off as one’s own”. it is potentially considered as the most prevalent form of scientific dishonesty discovered in research papers. the present review aims to provide a thorough account of plagiarism to build awareness about all dimensions of plagiarism.the key words “plagiarism”, “types”, “detection” and “conseq...

easily degradating and various isomeric forms of rapamycin (sirolimus) face the determination of this compound to many challenges. in this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (rp-hplc) method for rapamycin. separation was performed on a c8 column (mz, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...

a gradient reversed-phase high performance liquid chromatography (hplc) method was developed for the assay of cetrorelix acetate, a synthetic decapeptide with gonadotropin-releasing hormone (gnrh) antagonistic activity used in infertility treatment. the hplc method, which is used to determine cetrorelix in bulk and pharmaceutical dosage forms, was validated per ich guidelines. the chromatograph...

quick identification of vibrio cholerae in epidemics is   important, on the other hand; conventional methods are time-consuming and   costly. the aim of this study was to develop a rapid, inexpensive and high   sensitivity method for quick identification of vibrio cholerae . for   this purpose we designed a pcr detection based on magnetic nanoparticles for   identification of bacterial dna by p...

conclusions both task-related and resting fmri techniques have the potential to detect early brain dysfunction related to ad. however, the use of fmri in ad populations thus far has been limited to a relatively small number of research groups. results bold fmri is considered to reflect the joined synaptic activity of neurons through mri signal changes because of alterations in blood flow, blood...

from the winter 2002 to spring 2006, 126 moribund rainbow trout with clinical signs of external body haemorrhages around and within the oral cavity were sampled from 10 rainbow trout farms situated in the northwest and west of fars province, iran and examined for the detection and identification of yersinia ruckeri, the causative agent of enteric redmouth disease. fish kidneys were cultured ase...

background: canine parvovirus (cpv) infection is one ofthe most common causes of infectious gastroenteritis in dogs andis a highly contagious, often fatal disease. the original virus(cpv type 2) has had some mutations since its emergence andnew variants (cpv-2a, 2b and 2c) have been reported from manycountries all around the world. early diagnosis and treatment canprofoundly affect the disease ...

no report has been published on the economical losses associated with errors in oestrus detection in cows under iranian intensive dairy management. the following study was carried out to determine the accuracy of oestrus detection by farmers using artificial insemination in shiraz dairy farms. milk samples were collected from 179 holstein milking cows at the time of artificial insemination. mil...