Rhizoctonia solani isolates obtained from common beans (Phaseolus vulgaris L.) were included in an AG4 anastomosis group in accordance with hyphal anastomosis. In the subgrouping of AG4 isolates, PCR-RFLP patterns in the rDNA-ITS were used. After obtaining the genomic DNA belonging to R. solani AG4, an approximately 700 bp amplification product of the ITS1-5.8S-ITS2 region was obtained with PCR...

The culture conditions for the enhanced mycelial growth of Ramaria botrytis was investigated. The optimal temperature and pH for the mycelial growth were 24℃ and 5.0, respectively. It was shown that starch was best of several carbon sources in Czapek-Dox medium as a minimal medium for the enhanced mycelial growth. Organic nitrogen sources were better than inorganic ones for mycelial growth. The...

Garlic leaf blight caused by Stemphylium solani is a serious disease in vegetable-growing areas of China (28). Symptoms of the disease start as small white spots (1 to 3 mm) enlarging to elongated purple necrotic lesions along the vein, followed by blighting of the leaf tips. Similar symptoms were produced by culture filtrates of S. solani, and a strong correlation was also found between garlic...

Sakuranetin (1) is a rice flavanone-type phytoalexin. We have already reported that the metabolites from the detoxification of 1 by Pyriculariaoryzae are naringenin (2) and sternbin. In this study, we investigated whether the rice sheath blight fungus Rhizoctoniasolani, another major rice pathogen, can detoxify 1. The extract of R. solani suspension culture containing 1 was analyzed by LC-MS to...

An endophytic fungus, Fusarium sp. was isolated from yew bark of eastern Himalaya. Ethyl acetate extract from its fermentation broth displayed considerable antimicrobial activity against three Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis and Staphylococcus epidermidis), three Gram-negative bacteria (Klebsiella pneumoniae, Escherichia coli and Shigella flexneri) and two patho...

Propolis is a natural brownish by-product created by honey bees. Due to the antimicrobial components it contains, propolis has been widely tested against important fungal pathogens that cause agricultural damage. The diluent used in extraction of causes differences total phenolic compounds and properties propolis. Fusarium solani, division Ascomycota, infectious many cultivated crops such as pe...

Background. According to the World Health Organization antibiotic resistance is among top ten threats human health, food safety and development. Today has reached alarmingly high levels all over world. Meanwhile, increase in synthetic drugs' production led pathogenic mycobiota's rapid adaptation created chemicals, which have a narrow focus of application. That why modern biotechnology pharmacol...