The aims of this study were estimation of thyroxin status in dogs infected with B. canis, and determination of the association between azotaemia and concentrations of total thyroxin (TT4) and free thyroxin (FT4) in canine babesiosis. Concentrations of TT4 and FT4 were determined using an immunoassay in 23 dogs infected with B. canis (nine azotaemic dogs and 14 non-azotaemic dogs). Mann-Whitney ...

1. Stable urea isotopes can be used to study urea kinetics in humans. The use of stable urea isotopes for studying urea kinetic parameters in humans on a large scale is hampered by the high costs of the labelled material. We devised a urea dilution for measurement of the distribution volume, production rate and clearance of urea in healthy subjects and renal failure patients using the inexpensi...

A spectrophotometric flow-injection analysis (FIA) system for monitoring clinical hemodialysis is demonstrated. The role of a dialysate urea detector incorporated in this bioanalytical system is played by an optical flow-through biosensor based on Prussian Blue film with chemically linked urease forming a monomolecular layer of the enzyme. This pH-enzyme optode-FIA system is useful for the sele...

I N THE METHOD for the determination of blood urea imitrogeit described by us in Clinical Chemistry, 5, 617 (1959) a suli uric arsenic pentoxide solution is used for the reaction between diacetvl nionoxime and urea. Since the article was published tile manufacturer of time systeln* has recommended tile use of a ferric-alum-acid reagent developed by P. Paubiollsky and based on proposals by F. Mc...

lactatingdairy cows (n=177) fed with grass and corn silage ad libitum kept in pasture, were randomly assigned to evaluate how urea nitrogen in plasma and milk can be related to their pregnancy rate. blood and milk samples were collected on the artificial insemination (ai) day to evaluate plasma urea nitrogen (pun) and milk urea nitrogen (mun) as well as progesterone levels, excluding cows with ...

Dr. José Guiserix, Service de Néphrologie-Dialyse, Centre Hospitalier Alfred Isautier, BP 350 CHGSPT, F-97448 Saint-Pierre de la Réunion (France) 40 -i Acknowledgment We are indebted to Mr. Eric Woljung for assistance in the preparation of the manuscript. Dear Sir, Despite its limitations [1], the three-sample method is still widely used for blood recirculation estimation to detect underdialysi...

objective(s): renal ischemia reperfusion (ir) contributes to the development of acute renal failure (arf). oxygen free radicals are considered to be principal components involved in the pathophysiological tissue alterations observed during renal ir. the purpose of this study was to investigate the effect of co-administration of melatonin (mel) and erythropoietin (epo), potent antioxidant and an...

Using the general concept of a dry multilayer analytical element, we can change chemical procedures and configurations to assay several blood components. In the assay of serum urea nitrogen, urease in the reagent layer catalyzes the hydrolysis of urea. A semipermeable membrane excludes aqueous base, but allows ammonia to diffuse to an underlying indicator layer. For the amylase determination, t...

Zincon (2-carboxy-2'-hydroxy-5'-sulfoformazylbenzene) has long been known as an excellent colorimetric reagent for the detection of zinc and copper ions in aqueous solution. To extend the chelator's versatility to the quantification of metal ions in metalloproteins, the spectral properties of Zincon and its complexes with Zn(2+), Cu(2+), and Co(2+) were investigated in the presence of guanidine...

THE ONLY METHOD available at present for the routine determination of citrulline in blood is the one first described by Archibald (1). This method is based on the original observation of Fearon (2) that in strongly acid solution citrulline condenses with diacetylmonoxime (DAM) to produce a peach-colored complex. Since urea also reacts with DAM under these conditions, Archibald removed it by inc...