A systematic, comprehensive strategy that optimizes sample preparation and chromatography to minimize matrix effects in bioanalytical LC/MS/MS assays was developed. Comparisons were made among several sample preparation methods, including protein precipitation (PPT), liquid-liquid extraction (LLE), pure cation exchange solid-phase extraction (SPE), reversed-phase SPE and mixed-mode SPE. The inf...

BACKGROUND The number of children being diagnosed with type 1 diabetes mellitus (T1DM) is on the rise and has more than doubled in the past 10 years in Bahrain. Some studies have linked low vitamin D levels with an increased risk of diabetes. There are concerns regarding the variations in circulating 25(OH)D levels measured by different laboratories and by using different analytical techniques....

BACKGROUND The European DRUID (Driving under the Influence of Drugs, Alcohol And Medicines) project calls for analysis of oral fluid (OF) samples, collected randomly and anonymously at the roadside from drivers in Denmark throughout 2008-2009. To analyze these samples we developed an ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for detection of 29 drugs a...

An ultra-fast and improved analytical methodology based on microextraction by packed sorbent (MEPS) combined with ultra-performance LC (UPLC) was developed and validated for determination of (E)-resveratrol in wines. Important factors affecting the performance of MEPS such as the type of sorbent material (C2, C8, C18, SIL, and M1), number of extraction cycles, and sample volume were studied. Th...

Active pharmaceutical ingredients (APIs) can be assured to safe and effective with the help of stability-indicating procedures. An accurate comparison comprising utilization capillary zone electrophoresis (CZE) ultra-performance liquid chromatography (UPLC) for sensitive measurement ciprofloxacin (CPF) in presence its major photo-degradation product was conducted. The CZE UPLC working condition...

This study focused on the biological evaluation and chemical characterization of a new ingredient obtained by in vitro cell culture Rhus coriaria L. An plant method permits to cultivate short period time obtain extract with high safety profile for consumer, free from heavy metals, pesticides, aflatoxins, bacterial or fungal contamination. Through selection specific media, it was possible line c...

In this research article, a new chromatographic method ultra-performance liquid chromatography (UPLC) was developed for the quantification of ivermectin (IMT)-preziquantel (PRZQ) in two-component mixture and veterinary dosage form mixture. The separation studied drugs (IMT PRZQ) carried out on Waters Acquity ® BEH C18 column (50mm x 2.1 mm i.d., 1.7 m) using mobile phase consisting water-aceton...

Abstract Liensinine is a bisbenzyltetrahydroisoquinoline alkaloid extracted from lotus ( Nelumbo nucifera GAERTNER., Nelumbonaceae), especially in its embryo loti “Lien Tze Hsin” (green of mature seed). A rapid and simple UPLC-MS/MS method was developed to determine liensinine mouse blood application pharmacokinetic study. The samples were preprocessed by protein precipitation using acetonitril...

Different factors such as the genotype, environmental conditions, temperature stress, solar radiation and others can influence phytochemical status of plants. The concentration phenolic acids alkylresorciols (ARs) well their chemical composition biological activity have been determined in twelve winter wheat cultivars grown at eight European locations. This was first multi-location field trial ...