background: the use of igys in a variety of methods in different areas of research, diagnostics, medical application and biotechnology should be considered widely. objectives: development of antibodies against extra cellular domain of influenza m2 (m2e) protein in egg yolk of laying hens. methods: a fusion construct harboring c-terminal of bovine heat shock protein 70 (hsp70) and influenza m2e ...

two groups of antibodies against mouse resident alveolar and peritoneal macrophages have been raised individually in rabbits by immunization with whole cell suspensions of lavaged alveolar and peritoneal macrophages. the whole antisera reacted positively with neutrophils and lymphocytes. however, upon extensive absorptions with these cells the activity became restricted to macrophages. the immu...

Objective: To investigate possible immunological humoral correlates in newly diagnosed adult-onset generalized tonic-clonic epilepsy among Iranian patients before and after sodium valproate treatment.   Patients and Methods: 72 adult patients with newly diagnosed idiopathic generalized tonic-clonic epilepsy were recruited. Serum antinuclear antibodies (ANA), anti-cardiolipin antibodies (aCL), a...

So far, recombinant antigens of HIV-1, the etiologic cause of Acquired Immunodeficiency Syndrome (AIDS), have been widely used for the diagnosis and vaccine development. P17 or the matrix protein formed by the proteolytic cleavage of gag is strongly antigenic and is as conserved and immunogenic as p24. In some cases, antibodies to p17 are more prevalent than antibodies to p24 and the decline in...

Antibodies provide a suitable tool in fundamental research and their high affinity and specificity make them invaluable for diagnostic and therapeutic applications. A promising alternative to conventional antibodies are the heavy chain antibodies (VHH) of Camelidae having short length, high solubility and stability are preferred to other antibody derivatives. In this study, our goal was product...

Aim . To determine the presence or absence of specific IgG antibodies against SARS-CoV-2 antigens in outpatient clinic staff and to compare clinical immunological features from April August 2020. Materials Methods The control group comprised 386 employees Novosibirsk City Clinical Polyclinic №13.The determination blood serum was performed by using ELISA method. A real time method reverse transc...

background aspergillus fumigatus (af) is responsible for 90% of human infections. the lung is the predominate site of infection. it is able to colonize in respiratory tract of asymptomatic population and changed clinical features from noninvasive to invasive according to immuncomponent state of individuals. the aim of the present study was to determine the frequency of af immunoglobulin (igg) a...