1. The relationship between erythrocyte 2,3-diphosphoglycerate (2,3-DPG) and changes in blood pH and in oxygen affinity were studied in six patients treated with two dialysis techniques: conventional haemodialysis and ultrafiltration followed by conventional haemodialysis. 2. The decrease in erythrocyte 2,3-diphosphoglycerate and increase in pH after conventional haemodialysis may significantly...

To obtain high quality final products, biomolecules can be exposed in production lines to stress factors such as heating, stirring and multiple surfaces contact before their commercialization. These induce stability efficacy loss. In this work, the antibacterial activity of lysozyme (LSZ), a protein used antibiotic which acts by disrupting bacterial cell wall, was studied after two operations: ...

The value of the unbound drug fraction in plasma is a common characteristic required for drug discovery and development. The communication considers an important possibility that the unbound drug fraction at physiological temperature (37°C) could be substantially greater (more than 5 times) than the value measured by traditionally set ultrafiltration method with the incubation of drug plasma so...

UNLABELLED We treated persistent hemorrhage after cardiopulmonary bypass in a heart transplant recipient who had received anticoagulation with the direct thrombin inhibitor bivalirudin by a combination therapy aimed at reducing the plasma concentration of the thrombin antagonist (hemodialysis and modified ultrafiltration), increasing the concentration of thrombin at bleeding sites (recombinant ...

With the explosion of proteomics and protein engineering, modern medicine and life science has taken a strong foothold in bench-scale laboratories, both academic and industrial. A vital component of benchscale development of protein-based technologies is purification. Engineered proteins often undergo a stage of the purification process termed ultrafiltration separation. This involves the separ...

I. T. OLIVER AND W. A. RAWLINSON I955 the effect of the free aldehyde group. The Soret band of the oxime of the copper complex, in addition to being shifted to shorter wavelengths, is much more intense than that of the copperporphyrin a itself. The similarity of the absorption spectra of copper-porphyrin a and pyridine-haemochrome a, and their difference from the usual two-banded systems of mos...

When a membrane filtration process such as ultrafiltration is used a fluxand yield-decline can be observed. The causes are i) concentration polarization (i.e. accumulation of retained solutes, reversibly and immediately occurring) and ii) fouling phenomena such as adsorption, pore-blocking and deposition of solidified solutes, a long-term, and more or less irreversible process. The result of bo...