A single-base mutation assay using the non-crosslinking aggregation of neutravidin-modified polystyrene nanoparticles is described. This method requires only two steps: hybridization of biotinylated probe and sample DNAs, and then mixing with neutravidin-modified nanoparticles. The aggregation was detected within 20 min in total. A combination of the DNA non-crosslinking aggregation and biotin-...

Marshall Test design methods are used to design TLA modified asphalt mixture and base asphalt mixture respectively, and their pavement performance is tested. The results showed that there is a transforming coefficient of 1.15 between OAC of 40% TLA modified asphalt mixture and that of base asphalt mixture, and ultimate gradation of the TLA modified asphalt mixture is finer than previous gradati...

Soap Lake is a meromictic, alkaline (∼pH 9.8) and saline (∼14-140 g liter(-1)) lake located in the semiarid area of eastern Washington State. Of note is the length of time it has been meromictic (at least 2000 years) and the extremely high sulfide level (∼140 mM) in its monimolimnion. As expected, the microbial ecology of this lake is greatly influenced by these conditions. A bacterium, Halanae...

Base 37 in tRNA, 3'-adjacent to the anticodon, is occupied by a purine base that is thought to stabilize codon recognition by stacking interactions on the first Watson-Crick base pair. If the first codon position forms an A.U or U.A base pair, the purine is likely further modified in all domains of life. One of the first base modifications found in tRNA is N6-isopentenyl adenosine (i6A) present...

BACKGROUND Occipitocervical instability may be attributed to congenital, bony/ligamentous abnormalities, trauma, neoplasm, degenerative bone disease, and failed atlantoaxial fixation. Indications for occipitocervical fixation include the prevention of disabling pain, cranial nerve dysfunction, paralysis, or even sudden death. METHODS The screw trajectory for the modified transcondylar screw (...

the performance of selective media ltb, (leauryl tryptose broth), mmgm (minerals modified glutamate medium), bgbb (brilliant green bile broth), for the detection of e.coli in water in water, were compared and the following result were obtained: the mmgm found to be unsuitable as confirmatory medium for the detection of e.coli at 44 c, due to the occurrence of an unacceptable number of the false...

OBJECTIVE AI/RHEUM is a multimedia expert system developed originally to assist in the diagnosis of rheumatic diseases in adults. In the present study we evaluated the usefulness of a modified version of this diagnostic decision support system in diagnosing childhood rheumatic diseases. METHODOLOGY AI/RHEUM was modified by the addition of 5 new diseases to the knowledge base of the system. Cr...

Modified by the pretreated model with collagen under the crosslinking effect of glutaraldehyde, the moisture absorption of modified samples increased by 26.34% and 42.6%. The test results of SEM, AFM and DSC show that there was coating on the fibers modified with collagen, and the relative average roughness of the modified fabric decreased. Modification with collagen also affected the thermal p...

To investigate the mechanisms of spontaneous mutation in the Escherichia coli mutD5 mutator strain, 502 mutations generated in this strain in the N-terminal part of the lacI gene were sequenced (i-d mutations). Since the mutator strength of this strain depends on the medium in which it grows, mutations were analyzed in both minimal medium (moderate mutator activity) and rich medium (high mutato...

The use of molecular virology is described. Emphasis is placed on molecular biology, especially methods involving gene technology. The respective techniques allow characterisation of viruses by molecular cloning and nucleotide sequencing as well as the development of new diagnostic tools and vaccines. Genetically engineered live viruses serve as examples to study potential risks of vaccines der...