Recently, allergy to titanium has been reported. However, the patch test reagent for titanium is not standardized yet. The form and density of the patch test reagent for titanium was changed and examined in a group of normal subjects and a group with skin disease. The following conclusions were obtained. 1. A reagent which was composed of pure titanium powder and vaseline was not preferable bec...

A new reagent for the oxidative cleavage of DNA, (1,4,7-trimethyl-1, 4,7-triazacyclononane)iron(III) chloride was recently introduced. We have determined the utility of this reagent for detecting protein-DNA interactions within two types of complexes. Interestingly, we find that the rates of DNA cleavage by this reagent are differentially affected by the two classes of protein-DNA interactons s...

In this work, the effect of Fenton's reagent on the degradation of residual Kraft black liquor was investigated. The effect of Fenton's reagent on the black liquor degradation was dependent on the concentration of H2O2. At low concentrations (5 and 15 mM) of H2O2, Fenton's reagent caused the degradation of phenolic groups (6.8 and 44.8%, respectively), the reduction of reaction medium pH (18.2%...

METHODS: The plasmid expression vectors employed included pCMVLuc carrying the Firefly luciferase gene and pCMVhIGF-I carrying a human insulin-like growth factor I (hIGF-I) cDNA as control treatment [1-3]. Bone marrow aspirates were obtained from the femurs of normal donors during knee endoprothesis and MSCs were isolated and expanded in culture using standard protocols [4-6]. Briefly, the aspi...

This article is devoted to the use in concrete of polymeric reagent POLY-ANS, developed based on production waste polyacrylonitrile fibers. The results effect capillary absorption concrete, which contributes provision high resistance cement saline environments, are given.

Here we report a modified peptide reagent useful for the rapid, native elution of protein complexes containing a Protein-A-tagged component. We tested this reagent for the elution of tagged endogenous protein complexes from yeast (Nup53p/Nup170p dimer; Nup1p/Kap95p/Kap60p trimer; pentameric GINS complex) and bacteria (RNAP holoenzyme). The majority of the affinity-isolated material is released ...