Evidence suggests that the herpes simplex virus regulatory protein ICP27 mediates the nuclear export of viral transcripts; however, the extent of this activity during infection is unclear. ICP27 is required for efficient expression of the long, leaky-late UL24 transcripts, but not for that of the short, early UL24 transcripts. We found that infection by an ICP27-null mutant resulted in undetect...

While most transcriptome analyses in high-throughput clinical studies focus on gene level expression, the existence of alternative isoforms of gene transcripts is a major source of the diversity in the biological functionalities of the human genome. It is, therefore, essential to annotate isoforms of gene transcripts for genome-wide transcriptome studies. Recently developed mRNA sequencing tech...

The objective was to investigate effects of progesterone (P4) dose on abundance luteinizing hormone receptor (LHCGR), aromatase (CYP19A1), 3β-hydroxysteroid dehydrogenase (HSD3B1), and other steroidogenic mRNA transcripts in granulosa cells from dominant follicles. Nellore heifers were assigned one six groups: new, first-use controlled internal drug release device (CIDR1) inserted for 5 days (L...

We examined the transcription of six cellular oncogenes during the process of compensatory growth in rat liver after partial hepatectomy. We have previously reported that transcripts of c-rasH are elevated during regenerative growth of the liver. We now report that transcripts of c-rasK and c-myc genes are significantly elevated after partial hepatectomy, whereas transcripts of c-abl and c-src ...

Transcription in mammalian cells can be assessed at a genome-wide level, but it has been difficult to reliably determine whether individual transcripts are derived from the plus or minus strands of chromosomes. This distinction can be critical for understanding the relationship between known transcripts (sense) and the complementary antisense transcripts that may regulate them. Here, we describ...

There are abundance of transcripts that code for no particular protein and that remain functionally uncharacterized. Some of these transcripts may have novel functions while others might be junk transcripts. Unfortunately, the experimental validation of such transcripts to find functional non-coding RNA candidates is very costly. Therefore, our primary interest is to computationally mine candid...

Given the importance of transcriptome analysis in various biological studies and considering thevast amount of whole transcriptome sequencing data, it seems necessary to develop analgorithm to assemble transcriptome data. In this study we propose an algorithm fortranscriptome assembly in the absence of a reference genome. First, the contiguous sequencesare generated using de Bruijn graph with d...

Absorption of arsenic (As) by plant root is an essential activity that bridges the soil As concentration and physiological responses which can be monitored soon after exposure to As. In the present study, physiological responses to short exposure to arsenite (AsIII) was compared in two indigenous rice cultivars one adapted to temperate and humid (TH) and the second one to warm and humid (WH) cl...

the sporozoites of theileria annulata invade bovine mhc ii cells, where they differentiate into schizonts. the later can immortalize and induce fundamental changes in their host cells. live attenuated vaccine is an important way of controlling t. annulata infection of cattle. production is by prolonged cultivation of macroschizont-infected cells. the mechanisms underlying this transformation ar...

Miracle fruit ( Synsepalum dulcificum ) is the botanical source of miraculin, a natural, noncaloric sweetener. plants have bush-like architecture and produce multiple flushes attractive red berries each year. The consist large seed, opaque pulp, brilliant peel. pulp contains glycoprotein, that binds to tongue’s sweet receptors induces conformational change in response acidic stimuli. Thus, stro...