PURPOSE To determine whether adenovirus vectors are capable of transferring a foreign active protein to the perfused anterior segment of the human eye. METHODS Primary cultures from the human trabecular meshwork tissue were exposed to replication-deficient adenovirus Av1LacZ4 carrying the reporter beta-galactosidase gene driven by the Rous Sarcoma Virus promoter. Anterior segments of six pair...

PURPOSE To develop and characterize a model of pressure-induced optic neuropathy in rats. METHODS Experimental glaucoma was induced unilaterally in 174 Wistar rats, using a diode laser with wavelength of 532 nm aimed at the trabecular meshwork and episcleral veins (combination treatment group) or only at the trabecular meshwork (trabecular group) through the external limbus. Intraocular press...

Abstract This study aims to investigate the beneficial effects of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) on trabecular meshwork under oxidative stress and predict candidate genes associated with this process. Trabecular were pretreated BMSC-derived for 24 h, exposed 0.1 mM H 2 O 6 h. Survival rate was measured CCK-8 assay. Production intracellular reactive oxygen speci...

In the normal eye, the main resistance to aqueous outflow is in the outermost region of the trabecular meshwork, which is adjacent to and forms the inner wall of Schlemm's canal. In primary open-angle glaucoma, there is increased resistance to outflow, and we assume that the abnormal additional resistance is in the same location. Examinations of pathological anatomy show scarring and sclerosis ...

Operations on the trabecular meshwork in order to reduce raised intraocular pressure are receiving increasing prominence. Redmond Smith (i960) opened the trabecular tissue by passing a nylon thread along the canal of Schlemm via an ab externo incision, and by a further incision and by picking up the distal end of the thread, he ruptured the trabecular meshwork by pulling on the thread like a bo...

The authors present the results of 2 years' observation of 47 eyes operated with diathermo-trabeculotomy ab externo. This technique, based on a diathermic effect in association with trabeculotomy ab externo, allows an ample opening of Schlemm's canal and of the trabecular meshwork, with normalisation of intraocular pressure and outflow facility, for a period of up to 2 years, in open-angle and ...

With advancing age, alterations in the trabecular meshwork of human eyes include hyalinization, proliferation of long-spacing collagen, and reduction in the number and size of the vacuolated cells in the endothelial lining of the trabecular wall. These altera­ tions are thought to lead to an increase in the resistance to aqueous outflow. However, in aging persons whose eyes are healthy, pres­ s...

PURPOSE The primary cause of intraocular pressure (IOP) elevation in pseudoexfoliation glaucoma is obstruction of the intertrabecular spaces by exfoliation material. The aim of the current study was to evaluate the efficacy of a novel surgical approach--trabecular aspiration--for the management of pseudoexfoliation glaucoma. METHODS Twelve patients characterized by medically uncontrolled IOP ...

&gl~$g. Anterior segment perfusion povides a tool for determining flow in the trabecular outflow pathway only, contrary to perfusion of whole eyes in which 10-3046 of the outflow comes on account of the uveoscleral pathway. Since disposed human eyes are not abundantly available, especially not as a pair, it would be convenient to develop a model in which one half of an anterior segment could se...

The iridocorneal angle and inner layers of the trabecular meshwork in human fetal eyes were studied by scanning electron microscopy. Tissue from 32 eyes of 17 fetuses with a gestational age from 12 to 22 weeks were investigated in order to determine the morphological changes in the cellular lining of the anterior chamber angle recess during development. The findings indicate that, although hexa...