Many neurological and psychiatric disorders are genetic (e.g. Huntington's chorea), have a genetic component (e.g. Alzheimer's disease, bipolar depression) or are thought to involve alterations in gene expression (e.g. Pick's disease, Alzheimer's disease). It would, therefore, be useful to examine gene expression in post-mortem human brains. For this reason we have assessed the stability of bra...

Gene expression studies require analysis of RNA, but isolation of total RNA from very small samples by traditional methods can be difficult and inefficient. The Absolutely RNA microprep kit provides a convenient method for isolating total RNA from small numbers of cells such as those harvested by laser capture microdissection (LCM). The protocol includes binding of RNA to a solid support, thus ...

In order to investigate some of the cytochemical processes involved in interphase growth and culminating in cell division, a combined autoradiographic and microphotometric study of nucleic acids and proteins was undertaken on statistically seriated cells of Vicia faba root meristems. Adenine-8-C(14) and uridine-H(3) were used as ribonucleic acid (RNA) precursors, thymidine-H(3) as a deoxyribonu...

background & objectives: antisense peptide nucleic acids (pna) that target growth essential genes show potent bactericidal properties without cell lysis. we considered the possibility that whether pna treatment influence the bacteria total nucleic acids content and apply approach to develop a new delivery system to dendritic cells (dcs). dcs are the most potent antigen presenting cells in t...

The importance of high quality sample material, i.e. non-degraded or fragmented RNA, for classical gene expression profiling is well documented. Hence, the analysis of RNA quality is a valuable tool in the preparation of methods like RT-qPCR and microarray analysis. For verification of RNA integrity, today the use of automated capillary electrophoresis is state of the art. Following the recentl...

BACKGROUND Retrospective studies of archived human specimens, with known clinical follow-up, are used to identify predictive and prognostic molecular markers of disease. Due to biochemical differences, however, formalin-fixed paraffin-embedded (FFPE) DNA and RNA have generally been extracted separately from either different tissue sections or from the same section by dividing the digested tissu...

The effects of DNA interacting drugs on: (1) total RNA synthesis catalyzed by E. coli and T7 RNA polymerase; (2) synthesis of the initiating dinucleotide (pppApU) by E. coli RNA polymerase ("abortive initiation"); (3) elongation of RNA chains synthesized by T7 RNA polymerase on pT7-7 plasmid DNA bearing T7 RNA polymerase promoter phi 10 with human Cu/Zn superoxide dismutase coding sequence, (4)...

diffuse large b-cell lymphoma is the most common type of non-hodgkin lymphoma. micrornas (mirnas) are endogenous small rna, which can regulate gene expression at the post-transcriptional level. mirna profiling has shown a great potential as novel diagnostic and prognostic biomarkers. the present study was performed at the nemazee teaching hospital (shiraz, iran) from 2011 to 2013.the aim of thi...

among the viruses infecting honey bees, chronic bee paralysis virus (cbpv) is known to induce significant losses in honey bee colonies. cbpv is an unclassified polymorphic single stranded rna virus. using rt-pcr, the virus infections in honey bees can be detected in a rapid and accurate manner. bee samples were collected from 23 provinces of iran, between july-september 2011 and 2012. a total o...