PCR and real-time PCR primers for the 18S rRNA gene of rumen protozoa (Entodinium and Dasytricha spp.) were designed, and their specificities were tested against a range of rumen microbes and protozoal groups. External standards were prepared from DNA extracts of a rumen matrix containing known numbers and species of protozoa. The efficiency of PCR (epsilon) was calculated following amplificati...

background: encephalitozoon cuniculi infects a wide range of homoeothermic animals, including man. complications due to this microsporidian have been reported only in immunocompromised patients. reports on e. cuniculi in immunocompetent humans are lacking, most probably, because it is not linked to any clinical manifestations in such hosts. the present work was carried out with the aim of study...

Rumen contents were collected from ten adult female blesbok, five from a mine area with only acid drinking water available and five from a control group consuming normal, non-polluted drinking water. The mean concentration of total protozoa in the normal water group was almost double that in the acid drinking water group, 24.9 x 10(3) versus 14.7 x 10(3). Percent of Entodinium was higher and Di...

THE comparative investigations of Eggleton and Eggletonw, Meyerhof and Lohmanndi), Meyerhof (io), and Needham, Needham, Baldwin and Yudkin (n) have established a considerable body of information concerning the distribution of the phosphagens in the animal kingdom, and the character and implications of this knowledge have recently been analysed by Needham and Needham (13). But from all discussio...

background: conventional wastewater treatment is a combination of physical and biological processes designed to remove organic matter from solution. waste sludge drawn from these processes is thickened and processed for ultimate disposal wastewater contains various kinds of infectious agents, such as bacteria, viruses, fungi and parasites. correct treatment in wastewater treatment plants is imp...

Merino ewes (n 108) joined to a single sire were allocated into three flocks, with ewes in one flock being chemically defaunated in the second month of gestation. Single lambs born to defaunated ewes (BF lambs) were heavier at birth and at weaning than lambs born to faunated ewes (F lambs). After weaning, all BF and F lambs were individually housed then half of the F lambs were chemically defau...

In a preliminary study, the addition of antibiotics was shown to reduce bacterial concentrations in Entodinium exiguum cultures by more than 99% in 4 h, whereas the protozoal population was apparently unaffected. Using this procedure, the growth and amylolytic capability of Entodinium exiguum, in the presence or absence of live bacteria, was studied in vitro. Treatments for Trial 1 were protozo...

in the urine of a patient with chronic prostatitis, renal microlithiasis and acute cystitis we found the ciliate protozoa colpoda spp . , both in vegetative and cystic form. the entry point was most likely the urinary tract. keeping in mind that only four more cases of colpoda spp . existent in human urine have already been described, and that in the case of our patient the ciliate was present ...

Studies on Legionella show a continuum from environment to human disease. Legionellosis is caused by Legionella species acquired from environmental sources, principally water sources such as cooling towers, where Legionella grows intracellularly in protozoa within biofilms. Aquatic biofilms, which are widespread not only in nature, but also in medical and dental devices, are ecological niches i...

The article deals with the problem of studying structures and evolutions ecological chains at level microorganisms using mathematical computer models. Several models have been constructed based on laws biological kinetics. Computer experiments were developed these Mathematical describing dependence concentration bacterial biomass, substrates protozoa time created. With help Mathcad package, gra...