Tomato spotted wilt virus (TSWV) has one negative sense (L) and two ambisense (M and S) RNAs. The lack of a tractable TSWV reverse genetics system has impeded direct demonstration of gene function and prevented the usual types of pathogen characterization. The TSWV NSm (non-structural) protein has been presumed to be the viral movement protein (MP) based on a few lines of indirect evidence. We ...

The present investigation was carried out during 2017 at the Department of Plant Pathology, Dr YS Parmar University Horticulture and Forestry, Nauni, Solan. objective study to understand prevalence bacterial wilt tomato as influenced by edaphic factors. Periodic survey were undertaken crop season in major growing regions incidence ranged from 10.00% 84.02%. 20 days old seedlings inoculated with...

Bacterial wilt caused by R. solanacearum is a constraint that limits the production of solanaceous in Côte d'Ivoire. The genetic control, which more widely used, comes up against and phenotypic variability strains. objective this study was to screen commercial traditional varieties Solanaceae Ivorian strains different phylotypes assess their spectrum resistance disease. level virulence five eva...

Monitoring of pathogenic strains of Fusarium oxysporum (Fox), which cause wilt and rots on agricultural and ornamental plants, is important for predicting disease outbreaks. Since both pathogenic and non-pathogenic strains of Fox are ubiquitous and are able to colonize plant roots, detection of Fox DNA in plant material is not the ultimate proof of an ongoing infection which would cause damage ...

Twenty tomato spotted wilt virus (TSWV) isolates were serologically compared in ELISA employing five different procedures using a rabbit polyclonal antiserum against nucleocapsid proteins (NuAbR) and mouse monoclonal antibodies (MAbs), two directed to nucleocapsid proteins (N1 and N2) and four directed to glycoproteins G1 to G4. All the antisera were raised against TSWV-CNPH1. The 20 isolates w...

A model for the maturation of tomato spotted wilt virus (TSWV) particles is proposed, mainly based on results with a protoplast infection system, in which the chronology of different maturation events could be determined. By using specific monoclonal and polyclonal antisera in immunofluorescence and electron microscopy, the site of TSWV particle morphogenesis was determined to be the Golgi syst...