Methyltransferase RlmJ uses the cofactor S-adenosylmethionine to methylate the exocyclic nitrogen N6 of nucleotide A2030 in 23S rRNA during ribosome assembly in Escherichia coli. RlmJ with a C-terminal hexahistidine tag was overexpressed in E. coli and purified as a monomer using Ni(2+)-affinity and size-exclusion chromatography. The recombinant RlmJ was crystallized using the sitting-drop vapo...

The article examines the dynamics of culture as a result self-unfolding and self-knowledge being, agent which is person who both an actor himself, his own creating shell "second" screen (S. Zizek), stage on action unfolds, practice cognition being himself (including part being). There folding triad man culture, correlating with well-known formula Lacan: Real, Imaginary, Symbolic. Within boundar...

abstract the investigation of habermas’s political ideas of philosophical perspective and measuring his philosophical principles ratio, which is the deliberative democracy, is a purpose of this study that will be followed.  on the one hand, the importance of this issue is this that represents the new readings of democratic standards and on the other hand, consolidates one type of the democracy ...

Grassmann’s epistemological insights have attracted less attention than his mathematical results. Mathematicians have considered him as a precursor of modern mathematical theories – vector algebra, exterior algebra, Clifford algebra, and geometric algebra –, paying attention to the development of mathematical results rather than to the philosophical differences between Grassmann’s own project a...

D.G. Rossetti's works reveal how the poet, as an aestheticist through his pursuit of ideal, frees concept reality from pre-given limitations. He mainly creates open field renegotiation, even introducing alternative understanding real and authentic. In famous poems "The Blessed Damozel" Burden Nineveh," Rossetti applies intertextuality signifies potential dialectic poetry. His focus on aesthetic...

The philosophical programme associated with the discourse ethics of Jürgen Habermas has been widely discussed in the literature. The fact that Habermas has devoted a considerable part of his work to the elaboration of this philosophical programme indicates that discourse ethics can be regarded as a cornerstone of his communication-theoretic approach to society. In essence, Habermas conceives of...

Drawing largely on Aidoo’s (1970) play, Anowa, as well as lived experiences, I argue on the philosophical flaws of Ashcroft’s (2009) claim that there is no inherent link between language and culture. This essay subsequently explores the implication of my argument on some transformational domains of English in particular though it has obvious applicability to the role of colonial languages in ge...