نتایج جستجو برای: throughput sequencing

تعداد نتایج: 193236  

Journal: :AIDS research and human retroviruses 2013
Felicity Fitzgerald Kathryn Harris Ronan Doyle Dagmar Alber Nigel Klein

Microbial translocation (MT) from the gut is implicated in driving immune activation, increasing morbidity and mortality in HIV. We used bacterial 16S rDNA PCR, Sanger sequencing, and high-throughput sequencing to identify microbial DNA in the bloodstream of HIV-infected children in London, United Kingdom. Blood samples were collected from sequential children attending the HIV clinic at Great O...

Journal: :BioTechniques 2014
Kelly Quek Katia Nones Ann-Marie Patch J Lynn Fink Felicity Newell Nicole Cloonan David Miller Muhammad Z H Fadlullah Karin Kassahn Angelika N Christ Timothy J C Bruxner Suzanne Manning Ivon Harliwong Senel Idrisoglu Craig Nourse Ehsan Nourbakhsh Shivangi Wani Anita Steptoe Matthew Anderson Oliver Holmes Conrad Leonard Darrin Taylor Scott Wood Qinying Xu Peter Wilson Andrew V Biankin John V Pearson Nic Waddell Sean M Grimmond

Somatic rearrangements, which are commonly found in human cancer genomes, contribute to the progression and maintenance of cancers. Conventionally, the verification of somatic rearrangements comprises many manual steps and Sanger sequencing. This is labor intensive when verifying a large number of rearrangements in a large cohort. To increase the verification throughput, we devised a high-throu...

Journal: :Australasian Plant Disease Notes 2023

Abstract This is the first report of yambean mosaic virus and lettuce chlorosis in Australia. Both viruses were initially identified co-infecting Fabaceae weed, Calopogonium mucunoides collected from Umagico far north Queensland. Full coding sequences both obtained with high throughput sequencing confirmed RT-PCR Sanger sequencing. Lettuce has subsequently been several other species Queensland ...

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