There is a high demand for engineering thermostable enzymes in some industries; especially in paper industries to use environmental friendly enzymes instead of toxic chlorine chemicals. Hence, understanding protein attributes involved in enzyme thermostability is important. Herein, the most important protein features contributing to enzyme thermostability was searched by using data mining algor...

Abstract In this work we have applied the computational methodology based on Artificial Neural Networks (ANN) to kinetic study of distinct reaction mechanisms determine different types parameters. Moreover, problems ambiguity or equivalence are analyzed in set parameters systems when these from natures. The show possibility existence two possible parameter values that fit experimental data. det...

This review examines the fundamentals and challenges in engineering/understanding the thermostability of biological systems over a wide temperature range (from the cryogenic to hyperthermic regimen). Applications of the bio-thermostability engineering to either destroy unwanted or stabilize useful biologicals for the treatment of diseases in modern medicine are first introduced. Studies on the ...

The role of the C-terminal Leu300 in maintaining thermal stability of the neutral protease of Bacillus subtilis was investigated. From model building studies based on the three-dimensional structure of thermolysin, the neutral protease of B. thermoproteolyticus, it was concluded that this residue is located in a hydrophobic pocket composed of residues located in the C-terminal and the middle do...

Particle swarm optimization (PSO), as a novel evolutionary algorithm involved in social interaction for global space search, was firstly used in kinetic parameter estimation. Based on three developed nonlinear kinetic equations for bacterial cell growth, total sugar utilization and -mannanase production by Bacillus licheniformis under the support of a batch fermentation process, various PSO alg...

This study aimed to monitor the process parameters and to determine kinetics in composting of organic fraction of municipal solid waste (OFMSW) and poultry manure. The experiments were carried out with three different mixtures. The results showed that the mixture 60% OFMSW, 20% poultry manure, 10% mature compost and 10% sawdust provided the most appropriate conditions for composting process. Us...

Pyrolysis essentially is the thermal decomposition of organic matter under inert atmospheric conditions or in a limited supply of air, leading to the release of volatiles and formation of char. Modeling of pyrolysis implies the representation of the chemical and physical phenomena in a mathematical form. In this study, Differential Evolution (DE) algorithm is applied and global optimum set of k...