background and objectives: thermophilic bacteria are less studied but important group of microorganisms due to their ability to produce industrial enzymes. materials and methods: in this study, thermophilic bacteria were isolated from hot spring of tarabalo, india. a bacterium that could tolerate high temperatures was characterized by morphology, biochemistry and sequencing of its 16s rrna gene...

By comparing nucleic acid sequences determined for one of the most variable areas of 23S rRNA genes of 23 Campylobacter strains, we were able to identify regions specific for thermophilic Campylobacter strains. Oligonucleotide primers corresponding to these unique regions were synthesized and used in the polymerase chain reaction. One primer pair selectively detected all thermophilic Campylobac...

Protoplasts were produced from the thermophilic actinomycete Thermomonospora fusca and were regenerated to 0.1% of the direct count on regeneration agar. Recombination after protoplast fusion was demonstrated with drug-resistant mutants of T. fusca YX. A single thiostrepton-resistant colony was isolated after transformation of T. fusca YX with the streptomycete vector pIJ702, providing the firs...

BACKGROUND Thermophilic enzymes are often less active than their mesophilic homologues at low temperatures. One hypothesis to explain this observation is that the extra stabilizing interactions increase the rigidity of thermophilic enzymes and hence reduce their activity. Here we employed a thermophilic acylphosphatase from Pyrococcus horikoshii and its homologous mesophilic acylphosphatase fro...

Ribonucleases H from the thermophilic bacterium Thermus thermophilus and the mesophile Escherichia coli demonstrate a dramatic and surprising difference in their change in heat capacity upon unfolding (DeltaCp degrees ). The lower DeltaCp degrees of the thermophilic protein directly contributes to its higher thermal denaturation temperature (Tm). We propose that this DeltaCp degrees difference ...

There is an increased interest in using thermophilic bacteria for the production of bioethanol from complex lignocellulosic biomass due to their higher operating temperatures and broad substrate range. This review focuses upon the main genera of thermophilic anaerobes known to produce ethanol, their physiology, and the relevance of various environmental factors on ethanol yields including the p...

The removal of trace organic compounds (TrOCs) by a novel membrane distillation-thermophilic bioreactor (MDBR) system was examined. Salinity build-up and the thermophilic conditions to some extent adversely impacted the performance of the bioreactor, particularly the removal of total nitrogen and recalcitrant TrOCs. While most TrOCs were well removed by the thermophilic bioreactor, compounds co...

Exploration of the aquatic microbiota of several circum-neutral (6.0-8.5 pH) mid-temperature (55-85°C) springs revealed rich diversities of phylogenetic relatives of mesophilic bacteria, which surpassed the diversity of the truly-thermophilic taxa. To gain insight into the potentially-thermophilic adaptations of the phylogenetic relatives of Gram-negative mesophilic bacteria detected in culture...

Problem statement: The production of hydrolytic enzymes by T. aurantiacus has been performed under solid-state fermentations using lignocellulosic materials. The influences of the inoculum size and of the fermentation medium on the production of hemicellulases and cellulases were studied. Filtrates from the cultures were used to hydrolyze a pulp of sugarcane bagasse and the produced enzymes wer...

background: campylobacteriosis is one of the most commonbacterial intestinal disorders of humans in many countries.a wide range of domestic and wild bird species have been identifiedas natural reservoirs. objectives: the aim of this studywas to determine the campylobacter spp from pet birds andscreen the determined campylobacters for presence of virulencecytolethal distending toxin (cdt) genes....