The key property of Frost diagram has been confirmed using thermodynamic and linear algebra methods. On the basis of the thermodynamic data, the areas of thermodynamic stability of ruthenium species of different valence states as a function of pH for each degree of oxidation have been determined. Subsequently, based on the diagrams calculated for several values of pH, a narrow ΔpH value is dete...

Small hairpin RNAs (shRNAs) became an important research tool in cell biology. Reliable design of these molecules is essential for the needs of large functional genomics projects. To optimize the design of efficient shRNAs, we performed comparative, thermodynamic, and correlation analyses of ~18,000 miR30-based shRNAs with known functional efficiencies, derived from the Sensor Assay project (Fe...

using gaussian 03, software the thermodynamic functions such as gibbs free energy, g, enthalpy, h, and entropy, s, of alanine and valine amino acids were theoretically studied at different solvents. first, the density functional theory (b3lyp) level with 3-21g, 6-31g and 6-31+g basis sets were employed to optimization of isolated alanine and valine amino acids in the gas phase. moreover, vib...

Proteins from thermophiles are generally more thermostable than their mesophilic homologs, but little is known about the evolutionary process driving these differences. Here we attempt to understand how the diverse thermostabilities of bacterial ribonuclease H1 (RNH) proteins evolved. RNH proteins from Thermus thermophilus (ttRNH) and Escherichia coli (ecRNH) share similar structures but differ...

Benzohydroxamic acid is shown to be an unexpectedly good ligand for iron(iii) oxides, favouring surface attachment to the formation of trisbenzohydroxamato complexes, which are known to have very high thermodynamic stability in solution.

A hydrogen-bonded cyclic tetramer is assembled with remarkably high effective molarities from a properly designed dinucleoside monomer. This self-assembled species exhibits an impressive thermodynamic and kinetic stability and is formed with high fidelities within a broad concentration range.

thermodynamic study on the interaction of β-cd with poly ethylene oxide and poly acrylic acid was performed by isothermal titration calorimetry at 298k. when β-cd is added to the interpolymer complex, competition is created between host-guest and hydrogen bond. enthalpy of interaction between the β-cd and interpolymer complex was calculated using the extended solvation theory. p=1 shows that wi...

Kinetic and thermodynamic studies reveal that the property of significantly enhanced catalytic activity with colloidal stability is attributed to an efficacious means of tuning enzyme-substrate association by varying with the rate constants in the presence of functionalized gold nanoparticles.

The favorable biophysical attributes of non-antibody scaffolds make them attractive alternatives to monoclonal antibodies. However, due to the well-known stability-function trade-off, these gains tend to be marginal after functional selection. A notable example is the fibronectin Type III (FN3) domain, FNfn10, which has been previously evolved to bind lysozyme with 1 pM affinity (FNfn10-α-lys),...