Expression of platelet thromboxane receptors is transcriptionally increased during megakaryocytic differentiation stimulated by phorbol 12-myristate 13-acetate (PMA). We previously cloned and characterized the promoter region of the human thromboxane receptor gene and localized PMA-responsive elements to a region between 1.84 and 1.95 kilobase pairs (kb) 5' of the transcription initiation site ...

The involvement of protein kinase C (PKC) in the regulation of Na(+)-dependent and -independent hypoxanthine transport was investigated by exposing confluent monolayers of LLC-PK1 renal epithelia cells to the PKC activator, phorbol 12-myristate 13-acetate (PMA). Chronic exposure (> 2 h) of LLC-PK1 monolayers to 16 nM PMA resulted in approximately 75% inhibition of Na(+)-dependent hypoxanthine i...

Aims: In the NOD-like receptor (NLR) family, the pyrin domain containing 3 (NLRP3) inflammasome is closely related to the progression of atherosclerosis. This study aimed to assess the effects of curcumin on NLRP3 inflammasome in phorbol 12-myristate 13-acetate (PMA)-induced macrophages and explore its underlying mechanism. Methods: Human monocytic THP-1 cells were pretreated with curcumin for ...

THP-1 cells differentiated with phorbol 12-myristate 13-acetate (PMA) are widely used as a model for function and biology of human macrophages. However, the conditions used for differentiation, particularly the concentration of PMA and the duration of treatment, vary widely. Here we compare several differentiation conditions and compare the ability of THP-1 macrophages to interact with the facu...

Performance monitoring in the Annai tool environment for distributed-memory parallel computing systems is achieved through a flexible combination of different types of instrumentation. Latent instrumentation in the communication library and inserted in executables by the compilation system can be dynamically configured during program execution. Analysis of the subject program and knowledge of t...

Dear Editor, The O2-generating enzyme NADPH oxidase, plays a crucial role in host defense against microbial infection through the production of reactive oxygen species (ROS).1The multisubunit NADPH oxidase complex can be detected in vitro by the nitroblue tetrazolium test (NBT).2The NBT test is used for the diagnosis of chronic granulomatous disease.3 However, several factors, such as some cyto...

Authors: Steven Devos, Flanders MECHATRONICS Technology Centre, Leuven, Belgium Bert Stallaert, division PMA, Mech. Eng. Dpt., Katholieke Universiteit Leuven Gregory Pinte, Flanders MECHATRONICS Technology Centre Wim Symens, Flanders MECHATRONICS Technology Centre Paul Sas, division PMA, Mech. Eng. Dpt., Katholieke Universiteit Leuven Jan Swevers, division PMA, Mech. Eng. Dpt., Katholieke Unive...

This paper proposes a design for one-coil long-stroke permanent magnet actuator (PMA) applied to load breaker switch with novel mechanism applicable railways. As new railway overhead lines has double-insulation structure of the vacuum interrupter (VI) and disconnect (DS) higher insulation, control requires very long stroke. While traditional PMA implements stroke 10 30 mm, 120 mm. To do so, we ...

In Chinese hamster ovary (CHO) fibroblast cells the protein kinase C activating phorbol ester, phorbol myristate acetate (PMA), stimulates an increase in cell surface transferrin receptor (TR) expression by increasing the exocytic rate of the recycling pathway. The human TR expressed in CHO cells is similarly affected by PMA treatment. A mutant human TR in which the major protein kinase C phosp...