This short communication examines the question whether the experimental data presented in a study by Merrick et al. are of clinical relevance. These authors found that cannabidiol (CBD), a major cannabinoid of the cannabis plant devoid of psychotropic effects and of great interest for therapeutic use in several medical conditions, may be converted in gastric fluid into the psychoactive cannabin...

The objective of the current study was to assess the separate and combined effects of marijuana and alcohol on actual driving performance. Eighteen subjects were treated with drugs and placebo according to a balanced, 6-way, crossover design. On separate evenings they were given weight calibrated Delta(9)-tetrahydrocannabinol (THC) doses of 0, 100 and 200 &mgr;g/kg with and without an alcohol d...

Immune-mediated liver diseases including autoimmune and viral hepatitis are a major health problem worldwide. Natural cannabinoids such as Delta(9)-tetrahydrocannabinol (THC) effectively modulate immune cell function, and they have shown therapeutic potential in treating inflammatory diseases. We investigated the effects of THC in a murine model of concanavalin A (ConA)-induced hepatitis. Intra...

The multiple equilibria of the thermohaline circulation (THC: used here in the sense of the meridional overturning circulation) as function of the surface freshwater flux has been studied intensively following a Stommel paper from 1961. It is shown here that multistability and hysteresis of the THC also exist when the wind stress amplitude is varied as a control parameter. Both the Massachusett...

Apoptosis is programed cell death characterized by certain cellular changes and regulated by various gene products including Bcl-2 and caspase-1. The marijuana cannabinoid, Delta9tetrahydrocannabinol (THC), has been reported to suppress in culture the proliferation of splenocytes and increase the release of IL-1 from macrophages; however, the mechanisms of these effects remain unclear. Because ...

Steganography is an ancient art o r conveying messages in a secret way thal only Ihe receiver knows the existence of a message. So a fundamental requirement for a steganographic method is imperceptibility; this means that the embedded messages should not bc discernible to the human eye. There are two other requirements, one is to maximise the embedding capacity, and the other is security. Thc l...

The O-type forkhead domain transcription factor (FOXO) is involved in many biological processes such as aging, the oxidative stress response, and growth regulation. FOXO activity is tightly controlled within cells. In particular, growth factor signaling pathways and the oxidative stress response can both stimulate nuclear translocation of this transcription factor. Here, we show that tetrahydro...

The use of perfluorinated anhydrides coupled with perfluoroalcohols for the derivatization of cannabinoids has been well documented. Derivatization is used in the detection of cannabinoids using gas chromatography-mass spectrometry (GC-MS) with both electron impact ionization (EI) and negative chemical ionization (NCI). During method development for the analysis of cannabinoids in biological sa...

Although Delta(9)-tetrahydrocannabinol (THC) produces analgesia, its effects on nociceptive primary afferents are unknown. These neurons participate not only in pain signaling but also in the local response to tissue injury. Here, we show that THC and cannabinol induce a CB(1)/CB(2) cannabinoid receptor-independent release of calcitonin gene-related peptide from capsaicin-sensitive perivascular...

Drugs-of-abuse screening is becoming more prevalent, and a major technical and legal concern is that individuals may be mistakenly identified as drug usersbecause of a false-positive test result. Marijuana use can be detectedby screening a urine specimen with the Abbott (North Chicago, IL) TDx cannabinoid assay, which gives a positive reaction with several delta-9-tetrahydrocannabinol (THC) der...