3-Dehydroquinate synthase was purified to homogeneity from Escherichia coli. It was found to be a single polypeptide chain of M, = approximately 57,000. Reaction mixtures of pure enzyme and the substrate, 3deoxy-n-arabino-heptulosonic acid 7-phosphate, were incubated for short times and treated with NaB3H4. The resulting 3-deoxyheptonic acid 7-phosphate was degraded with sodium periodate, and f...

The objective of this study was to investigate the immune-enhancing activity of a high molecular weight fraction (HMF) of Cynanchum wilfordii in RAW 264.7 macrophages and the cyclophosphamide (CYC)-induced mouse model of immunosuppression. To identify the bioactive substances of HMF, a crude polysaccharide (HMFO) was obtained and treated with sodium periodate (an oxidation agent) or digested wi...

To improve the photo-catalytic degradation of salicylic acid, we reported the fabrication of ordered TiO2 nanotube arrays by a simple and effective two-step anodization method and then these TiO2 nanotubes treated in a methanol solution under UV light irradiation. The TiO2 nanotubes prepared in the two-step anodization process showed better photo-catalytic activity than TiO2 nanotubes prepared ...

The mosquito-active protein crystals produced by Bacillus thuringiensis subsp. israelensis contain covalently attached aminosugars which are critical for their larvicidal activity. The 50% lethal concentrations toward Aedes aegypti larvae were increased up to 10-fold by mild periodate treatment, up to 40-fold by forming the protein crystals in the presence of tunicamycin, and up to 7-fold by th...

A tritium derivative method for sequence analysis of polyribonucleotides is detailed, which is based on borotritide reduction of oligonucleotide dialdehydes generated by treatment of polyribonucleotides with alkaline phosphatase and excess periodate at pH 8 (borate buffer; no primary amine present in the reaction mixture). While neither phosphatase nor periodate possess any intrinsic exonucleas...

1. The reaction products of isoniazid with periodate-oxidized ribonucleosides and 5'-ribonucleotides have been characterized as the monohydrazones. 2. The stability, chromatographic and electrophoretic properties of the hydrazones are described. 3. (3)H-labelled isoniazid was shown to react with the 5'-linked terminal adenosine and cytidine groups of periodate-oxidized Escherichia coli transfer...

1951). These bodies usually occur with greatest frequency and staining intensity in smooth, virulent strains and diminish or disappear with colonial variation to rough, 0-inagglutinable forms. It was pointed out also that polar disposition of antigen would be more consistent with the observed pattern of end-to-end 0 agglutination (Pijper, J. Bact., 42, 345, 1941) than would an over-all surface ...

Under the conditions for oxidative degradation, with a 2-fold molar excess of periodate and cyclohexylamine at room temperature, *MP is degraded to inorganic phosphate but fails to release the base. In this latter respect, the parent pseudouridine behaves similarly. Under more drastic conditions, with a prolonged treatment at 4.5” and a 6-fold molar excess of periodate and cyclohexylamine, \kMP...