Skin barrier properties are critical for maintaining epidermal water content, protecting from environmental factors, and providing first line of defense against pathogens. In this study, the effect a non-proteinogenic amino acid, L-4-Thiazolylalanine (L4), on important aspect skin health was evaluated by in vitro vivo biomarkers. 3D human models were treated with 0.3% L4. After 48h, RNA samples...

In psoriasis (PSO) and atopic dermatitis (AD), the complex inflammatory milieu drives epidermal disease hallmarks that are associated to skin inflammation. The current lack in understanding on how this, often patient-specific, signature translates endotypes hampers development of translational models personalized use targeted drugs. We aimed identify optimal cytokine combinations mimic phenotyp...

Atopic dermatitis (AD) is characterized by skin barrier dysfunction, increased type 2 immunity, and an altered microbiota with high Staphylococcus aureus abundance. These factors contribute to enhanced susceptibility viral infections. We hypothesize that both immunity (IL-4/13) S. colonization alter the epithelium make it highly permissive Using human keratinocytes (KCs), we observed state of d...

Mitral regurgitation (MR) is the most commonly encountered valvular heart disease in United States and increases with age.1Nkomo VT Gardin JM Skelton TN Gottdiener JS Scott CG Enriquez-Sarano M Burden of diseases: a population-based study.Lancet. 2006; 368 (doi:10.1016/S0140-6736(06)69208-8.): 1005-1011Google Scholar There are two broad categories: degenerative mitral (DMR), caused by myxomatou...

Abstract Background Tricuspid regurgitation (TR) has long been neglected due to limited therapeutic options. Within the past five years, transcatheter tricuspid valve edge-to-edge repair (T-TEER) become a valuable tool in treatment of TR besides diuretic medical therapy and surgery. Owing its novelty, data on long-term survival after T-TEER for relevant are sparse. Beyond that, there is uncerta...

Abstract Inflammatory bowel disease (IBD) is a widespread disease, affecting growing demographic. The treatment of chronic inflammation located in the GI-tract dependent on severity; therefore, IBD pyramid commonly applied. Animal experimentation plays key role for novel drug development; nevertheless, it ethically questionable and limited its throughput. Reliable valid vitro assays offer oppor...

Enhancing oral bioavailability of hydrophilic drugs by encapsulation in lipid-based nanocarriers, including Solid Lipid Nanoparticles (SLN), has been well documented. In this work, high molecular weight heparin was “insolubilized” an “ion-paring” approach, forming Chitosan/Heparin Polyelectrolyte Complexes (PEC) to promote its SLN. Hybrid PEC-SLN, heparin-loaded SLN (H-SLN) as naked PEC were pr...

Dietary fiber intake is associated with lower incidence and mortality from disease, but the underlying mechanisms of these protective effects are unclear. We hypothesized that β2→1-fructan dietary fibers confer protection on intestinal epithelial cell barrier function via Toll-like receptor 2 (TLR2), and we studied whether β2→1-fructan chain-length differences affect this process. T84 human int...

Acid reflux in the esophagus can induce esophageal painful sensations such as heartburn and noncardiac chest pain. The mechanisms underlying acid-induced esophageal nociception are not clearly understood. In our previous studies, we characterized esophageal vagal nociceptive afferents and defined their responses to noxious mechanical and chemical stimulation. In the present study, we aim to det...

Activation of cannabinoid receptors decreases emesis, inflammation, gastric acid secretion, and intestinal motility. However, the effects of cannabinoids on intestinal permeability have not yet been established. The aim of the present study is to examine the effects of cannabinoids on intestinal permeability in an in vitro model. Caco-2 cells were grown until fully confluent on inserts in 12-we...