The Diels–Alder cycloaddition is arguably the most powerful reaction for the construction of substituted six-membered rings from various 2p and 4p reactants. However, unactivated 2p and 4p systems either require very harsh conditions or fail to undergo cycloadditions. In many cases, transition metal catalysts facilitated chemical transformations that were inaccessible under thermal conditions a...

A two-stage "tandem strategy" for the synthesis of indoles with a high level of substitution on the six-membered ring is described. Benzannulation based on the reaction of cyclobutenones with ynamides proceeds via a cascade of four pericyclic reactions to produce multiply substituted aniline derivatives in which the position ortho to the nitrogen can bear a wide range of functionalized substitu...

A sensitive liquid chromatograph/tandem mass spectrometric technique (LC/MS/MS) was applied to determine aliphatic and aromatic carbonyl compounds in ambient air. Traces of the carbonyl compounds were sampled by passing through a Sep-Pak DNPH-silica cartridge. Their derivatives were thus eluted with acetonitrile, separated by reversed-phase liquid chromatography and determined by quadrupole tan...

A two-stage "tandem strategy" for the regiocontrolled synthesis of very highly substituted quinolines is described. Benzannulation based on the reaction of cyclobutenones or diazo ketones with N-propargyl-substituted ynamides proceeds via a cascade of several pericyclic reactions to generate multiply substituted aniline derivatives. In the second stage of the tandem strategy, triflate derivativ...

Allylating agents were explored for the asymmetric synthesis of α-allyl-α-aryl α-amino acids by tandem N-alkylation/π-allylation. Cross-metathesis of the tandem product was developed to provide allylic diversity not afforded in the parent reaction; the synthesis of homotyrosine and homoglutamate analogues was completed. Cyclic α-amino acid derivatives could be accessed by ring-closing metathesi...

Abbreviations: PTMs=post-translational modifi cations; PPIs=protein-protein interactions; SDS-PAGE= sodium dodecyl sulfate-polyacrylamide gel electrophoresis; 2D-PAGE=two-dimensional polyacrylamide gel electrophoresis; MS=mass spectrometry; ESI=electrospray ionization; MALDI=Matrix Assisted Laser Desorption Ionization; ESI-MS=electrospray ionization mass spectrometry; MALDI-MS=MALDI mass spectr...

Objectives: The role of cognitive process for postural control was shown in dual task studies. However, there was no definite evidence how verbal instructions influence the allocation of attention to postural control. This study determined whether young and elderly adult are able to deliberately control the resource allocation when performing a sensorimotor and cognitive task simultaneously and...

The use of domino and multicomponent reactions in asymmetric synthesis is constantly increasing nowadays. This allows for the synthesis of complex molecules in a single synthetic sequence, usually with high atom economy. Herein, we report three examples of new asymmetric tandem reactions recently developed in our laboratories, giving rise to new families of enantiomerically enriched fluorinated...

Microtubule-associated proteins (MAPs), guanine nucleotides, and reaction temperature have substantial effects on the interaction of taxol with purified tubulin in 0.1 M glutamate. Without MAPs, taxol induced tubulin to polymerize only if GTP was present and the reaction mixture warmed. GTP was hydrolyzed in tandem with polymerization in stoichiometric amounts, while GDP inhibited both polymeri...