This study was conducted to assess the effect of spice blends varying in salt and pepper concentrations on microbial quality Quanta: Ethiopian dried red meat. The experiment had seven treatments: 25% spices, salt, 50% (T1); 20% 55% (T2); 15% 60% (T3); 10% 65% (T4); 5% 70% (T5); 100% spices (without pepper), a positive control (T6); negative without any added ingredient (T7). Microbiological ana...

Background: Gene 1.7 of bacteriophagephage T7 encodes a nucleotide kinase Results: Gp1.7 dodecamers catalyze the reversible dTMP and dGMP kinase reactions Conclusion: Gp1.7 is a unique nucleotide kinase that does not require a divalent metal ion Significance: The unique nucleotide kinase of bacteriophage T7 supplies dTDP and dGDP for conversion to the nucleoside 5’-triphosphate and recycles the...

Removal of nucleoside triphosphates from extracts prepared from bacteriophage T7-infected Escherichia coli results in a stringent requirement for added ATP to form infective phage particles by in vitro packaging of bacteriophage T7 DNA. Optimal packaging efficiency was achieved at a concentration of about 1.25 mM. Other nucleoside triphosphates could be substituted for ATP, but none of the comm...

The majority of the phage-induced proteins made after T7 infection of Escherichia coli are tightly associated with the bacterial membrane. Many of these have been identified. Selective extraction of proteins from these membranes by the detergent Sarkosyl or the chaotropic agent guanidine-hydrochloride indicated that most of these proteins are an integral part of the cytoplasmic membrane and the...

We have developed a method for transferring exogenous DNA molecules into isolated mammalian mitochondria using bacterial conjugation. In general, we accomplish this by (i) inserting an origin of DNA transfer (oriT) sequence into a DNA construct, (ii) transforming the construct into an appropriate Escherichia coli strain and then (iii) introducing the mobilizable DNA into mitochondria through co...

Angiostatin is a potent anti-angiogenic protein. To examine the angiostatin-interacting proteins, we used the display-cloning method with a T7 phage library presenting human cDNAs. The specific T7 phage clone that bound to the immobilized angiostatin was isolated, and a novel gene encoding the displayed polypeptide on the isolated T7 phage was identified. The displayed angiostatin-binding seque...

Helicases that unwind DNA at the replication fork are ring-shaped oligomeric enzymes that move along one strand of a DNA duplex and catalyze the displacement of the complementary strand in a reaction that is coupled to nucleotide hydrolysis. The helicase domain of the replicative helicase-primase protein from bacteriophage T7 crystallized as a helical filament that resembles the Escherichia col...

Transcription from the commonly used GTP- initiating T7 class III promoter phi6.5 frequently produces heterogeneous RNA at both 3' and 5' ends. We demonstrate here that RNA transcripts from the T7 class II promoter phi2.5 have superior 5' homogeneity over those from the phi6.5 promoter, with comparable total RNA yields. The overall homogeneity of RNA transcripts is improved to different degrees...