Bacterial ribonuclease P contains a catalytic RNA subunit that cleaves precursor sequences from the 5' ends of pre-tRNAs. The RNase P RNAs from Bacillus subtilis and Escherichia coli each contain several unique secondary structural elements not present in the other. To understand better how these phylogenetically variable elements affect the global architecture of the ribozyme, photoaffinity cr...

RNase P is an essential endonuclease in tRNA biogenesis, which generates the mature 5'-termini of tRNAs. Most forms of RNase P are ribonucleoproteins, i.e., they consist of an essential RNA and protein subunits. The catalytic function of ribonucleoprotein RNase P enzymes resides entirely in the RNA subunit. Its high structural and functional diversity among representatives of a vast variety of ...

Bacterial ribonuclease P RNA ribozyme can do the hyperprocessing reaction, the internal cleavage reaction of some floppy eukaryotic tRNAs. The hyperprocessing reaction can be used as a detection tool to examine the stability of the cloverleaf shape of tRNA. Until now, the hyperprocessing reaction has been observed in the heterologous combination of eukaryotic tRNAs and bacterial RNase P enzymes...

RATIONALE AND OBJECTIVES To assess the feasibility of quantitative T2* mapping at 3.0 T for prostate cancer detection and to investigate the use of T2* values to characterize tumor aggressiveness, with whole-mount step-section pathologic analysis as the reference standard. MATERIALS AND METHODS Prostate multiecho T2* was performed in 55 consecutive patients with prostate cancer using a multis...

We present the open-source tool T2, the first public release from the TERMINATOR project [8]. T2 has been extended over the past decade to support automatic temporal-logic proving techniques and to handle a general class of user-provided liveness and safety properties. Input can be provided in a native format and in C, via the support of the LLVM compiler framework. We briefly discuss T2’s arch...

This White Paper provides an overview of major techniques used in DVB-T2 and their impact on service planning and operation of this new transmission technology.