BACKGROUND To evaluate the impact of the introduction of prenatal screening on time of detection and pregnancy outcome for trisomy 21 (T21), trisomy 18 (T18) and trisomy 13 (T13). METHODS We performed a retrospective, population-based cohort study in the Northern Netherlands including 503 trisomy cases born between 2005 and 2012. Screening tests and invasive procedures, timing of diagnosis an...

We present transcriptome analyses of primary cultures of human fetal cells from pregnancies affected with trisomy 21 (t21) and trisomy 13 (t13). Pooled mRNA samples from t21 and t13 cases were used for comparative hybridizations to cDNA arrays with pooled mRNA from normal cells. When the array cDNAs were grouped by chromosomal location the relevant trisomic chromosome could be clearly identifie...

BACKGROUND Reliable individual risk calculation for trisomy (T) 13, 18, and 21 in first-trimester screening depends on good estimates of the medians for fetal nuchal translucency thickness (NT), free β-subunit of human chorionic gonadotropin (hCGβ), and pregnancy-associated plasma protein-A (PAPP-A) in maternal plasma from unaffected pregnancies. Means and SDs of these parameters in unaffected ...

The development of a T7 RNA polymerase (T7 RNAP) expressing cell line i.e. BSR T7/5 cells marks an improvement reverse genetics for the recovery recombinant Newcastle disease virus (rNDV). is developed by transient transfection plasmid encoding RNAP gene rNDV rescue. However, expression decreases gradually over multiple passages and eventually hinders rescue rNDV. To address this issue, lentivi...

To determine the role of protein kinase Cdelta in mouse skin carcinogenesis, we have developed transgenic FVB/N mouse lines expressing in the epidermis an epitope-tagged protein kinase Cdelta (T7-PKCdelta) regulated by the human keratin 14 promoter. The untreated T7-PKCdelta mice displayed excessive dryness in the skin of the tail with a variable penetrance over time. Histologically, the tail s...

Background: Onfarm Testings were conducted to asses effect of TNAU Pulse wonder in pigeonpea variety LRG-52 ten farmer’s holdings kurnool district during kharif from 2018-19 2020-21. Methods: The experiment consisted 7 technology options (treatments). T1: consisting recommended dose fertilizers along with spraying pulse @ 5 kg ha-1 flowering and 15 days after 1st spray, T2: 75% RDF + at floweri...

Aims: The combination of various sources organic manures along with liquid is important to maintain nutrient availability crop and sustain higher level soil fertility. aim meet the demand parching sorghum as per requirement at different stages through solid which can give greater productivity crops.
 Study Design: experiment was laid in Randomized Complete Block Design 9 treatments replica...

The inability of T7 to develop in cells of Escherichia coli containing F(+) or substituted F' episomes is a result of the failure to synthesize late proteins; no in vivo translation of mRNA species synthesized by the T7 RNA polymerase occurs. Further experiments have been performed to measure the amount of late mRNA in T7-infected F'(PIF(+)) cells. (We have designated the property of phage inhi...

Aims: In order to assess the efficacy of several biorational techniques against brinjal mite, Tetranychus urticae Koch.
 Study Design: The study used a single component Randomized Complete Block Design (RCBD) with three replications.
 Place and Duration Study: experiment was conducted in central farm Sher-e-Bangla Agricultural University, Dhaka, Bangladesh from October 2021 February 2...