1. The effect of perfusion on the activities of hexokinase and lactate dehydrogenase was studied in the proximal half of the small intestine of fed and starved rats. 2. Perfusion of preparations from starved rats with a medium containing glucose caused a significant increase in hexokinase activity of the particle-free supernatant. The increase in activity was observed as early as 5min after the...

background: trichophyton verrucosum is a zoophilic fungus with a worldwide distribution. our aim was to investigate the proliferative effect of antigenic compounds of t. verrucosum on dermis fibro blasts and endothelial cells. methods: t. verrucosum was cultured in scc medium and was then transferred to a broth medium. surface antigens of this fungus were separated using the freeze and thaw met...

An important clinical feature of invasive Staphylococcus aureus disease is the tendency for infection to persist or relapse despite seemingly adequate antibiotic therapy. One mechanism by which this is believed to occur is through the emergence of antibiotic-resistant small colony variants (SCVs) [1]. When SCVs are isolated from individuals with S. aureus infections, it is common to simultaneou...

A large proportion (60-85%) of the actin present in lysates of cultured baby hamster kidney (BHK) cells is monomeric at low temperature. All this G-actin is accounted for in the fractions obtained by high-speed centrifugation of the lysate in the presence or absence of non-ionic detergent; 98 (+/- 7)% of its reacts as monomer in the deoxyribonuclease assay. The fractions nevertheless possess ac...

The synthesis of palmitic and stearic acids by soluble proteins from avocado mesocarp has been examined. Evidence suggests that palmitate is made de novo and stearate by elongation of a C16 precursor. While the synthesis of palmitate from malonyl CoA is arsenite insensitive, the elongation reaction from palmitate to stearate is arsenite sensitive. The pattern of synthesis of the two acids is ne...

BACKGROUND Protein aggregation during monoclonal antibody (mAb) production can occur in upstream and downstream processing (DSP). Current methods to determine aggregate formation during cell culture include size exclusion chromatography (SEC) with a previous affinity chromatography step in order to remove disturbing cell culture components. The pre-purification step itself can already influence...

Extracellular proteins made by group A Streptococcus (GAS) play critical roles in the pathogenesis of human infections caused by this bacterium. Although many extracellular GAS proteins have been identified and characterized, there has been no systematic analysis of culture supernatant proteins. Proteins present in the culture supernatant of strains of serotype M1 (MGAS 5005) and M3 (MGAS 315) ...

the aim of this work was identified lactic floraas a traditional fermented food and then evaluates antimicrobial activities of some strains. a total of 140 gram-positive and catalase-negative isolates were subjected to grouping by physiological and biochemical tests and carbohydrates fermentation. based on the resultsthese 140 isolateswere dividedinto 9 groups. two or three isolated were select...

Abstract Recovery of phosphorus (P) from wastewater is a topic great interest. Besides being non-renewable resource, P discharge in receiving waters can trigger algae blooms. The present study aimed to quantify the removal two sites at treatment plant using calcined eggshell (CES) as adsorbent. CES was prepared raw shells 600 °C (CES600) and 800 (CES800). proved be an efficient material for rem...