A bottle-neck in recombinant antibody sandwich immunoassay development is pairing, demanding protein purification and modification to distinguish captor from tracer. We developed a simple pairing scheme using microliter amounts of E. coli osmotic shockates bearing site-specific biotinylated antibodies and demonstrated proof of principle with a single domain antibody (sdAb) that is both captor a...

Abstract Nylon composites are of evolving interest due to their good strength, toughness, and low coefficient friction. Various fillers like micro- nanoparticulates metals metal compounds were used enhance the mechanical tribological properties nylons for many years by researchers. In this paper, an overall understanding composites, filler materials, especially particulate application areas pol...

The recently developed evolutionary algorithm USPEX proved to be a tool that enables accurate and reliable prediction of structures. Here we extend this method to predict the crystal structure of polymers by constrained evolutionary search, where each monomeric unit is treated as a building block with fixed connectivity. This greatly reduces the search space and allows the initial structure gen...

The potential effectiveness of a silver nylon (SN) fabric as an antimicrobial agent was evaluated in a series of in vitro experiments. The results indicated that silver ions from the SN fabric penetrated 2 mm of agar and killed a challenge of 6.9 X 10(7) Pseudomonas aeruginosa, 4.1 X 10(7) Staphylococcus aureus, and 1.4 X 10(7) Candida albicans organisms per cm2 of SN fabric after 24 h of incub...

WATERTOWN, Aug. 3 — The Jefferson Chapter, Civil Service Employees Assn. will continue Its flght for board of supervisors participation in the plan generally gaining favor throughout the s tate—the pension payment reduction which is tantamount to a raise in pay. T1i8 Chapter plans to submit its recommendation to r.h® county board next month, probably asking for the total 8% payment benefit. Wat...