A sensitive high performance liquid chromathography (HPLC) analytical procedure was developed for the quantitive determination of trimethoprim (TM* and sulphamethoxazole (SM) in commercial dosage forms. C18 analytical column (stainless steel, 25 cm * 4.6 mm i.d.) was packed with 5-*m particles of the reversed phase material and used for assays. Mobile phase containing 0.025 M sodium phosphate...

Reports to the Committee on Safety of Medicines show that skin reactions are the commonest adverse reaction to trimethoprim; the committee has had a few reports of cholestatic jaundice due to trimethoprim and to trimethoprim-sulphamethoxazole. Manufacturers of trimethoprim (Duphar) have received four reports of hepatic reactions: hepatitis (two), jaundice (one), and cholestatic jaundice (one). ...

A randomised clinical trial of single dose trimethoprim against a seven day course of co-trimoxazole (trimethoprim/sulphamethoxazole) for the treatment of uncomplicated urinary tract infection was carried out in 106 children aged between 2 and 16 years. Of the 50 children with confirmed urinary tract infections who were followed up 48 hours after treatment with a single dose of trimethoprim all...

Infections caused by Chlamydia spp are an important cause of human disease, and the accuracy and reproducibility of antimicrobial susceptibility tests for these bacteria could have considerable clinical implications. We have developed a reverse transcriptase PCR (RT-PCR) based method to determine the antibiotic susceptibility of Chlamydia spp., and compared this with conventional tests using im...

Suspension dosage forms require the use of suspending agents in order to deliver a uniform dose of the active ingredient. The purpose of this study is to investigate the properties of a new plant gum as a cheap and effective natural polymer in the formulation of pharmaceutical suspensions. The gum obtained from the incised trunk of Cedrela odorata (family Meliaceae) was compared with hydroxypro...

The photostabilization of poly(vinyl chloride) (PVC) films having five Schiff’s bases derived from sulphamethoxazole has been investigated. The casting method was used to produce PVC films containing sulphamethoxazoles (0.5% by weight), in tetrahydrofuran. The photostabilization activities of five additives were determined by monitoring the hydroxyl, polyene and carbonyl indices with irradiatio...

We describe a case of Nocardia nova septic arthritis following a total knee replacement. A 55-year-old obese woman was admitted to hospital 5 months after knee surgery with a 3-week history of pain, swelling, and restricted mobility in her left knee but no preceding trauma/injury. 30 ml of cloudy joint fluid was aspirated and an arthroscopic examination showed extensive fibrin formation and syn...

CASE HISTORY A 43-yr-old native Brazilian female complained of a sore throat, recent onset of hoarseness, progressive pain and bilateral swelling of the neck during the past 6 months, when she presented at the University Hospital of Zurich (Zurich, Switzerland) for the first time. The patient had moved to Switzerland 5 yrs before, had been a heavy smoker for the past 20 yrs and coughed chronica...