According to experts, an individual's diet has a more significant impact on their health, around 90%, compared healthcare services, which only about 10%. Certain elements like iron, copper, zinc, molybdenum, and trace play crucial role in the biological functions of plants, animals, humans. The study was carried out through both field laboratory experimentation, following methodological manuals...

Most of the biological processes in living systems involve molecular adsorption and transport at biomembranes. It is highly desired to study the time-resolved transport kinetics through living cell membranes. In this thesis, an experimental means based on a nonlinear optical phenomenon, Second Harmonic Generation (SHG) has been demonstrated to detect the molecular adsorption and transport throu...

Although the stable oxygen isotope fractionation between dissolved sulfate ion ðSO 4 Þ and H2O (hereafter aðSO2 4 –H2OÞ) is of physico-chemical and biogeochemical significance, no experimental value has been established until present. The primary reason being that uncatalyzed oxygen exchange between SO 4 and H2O is extremely slow, taking 10 5 years at room temperature. For lack of a better appr...

Electron detachment dissociation (EDD) has previously provided stereo-specific product ions that allow for the assignment of the acidic C-5stereochemistry in heparan sulfate glycosaminoglycans (GAGs), but application of the same methodology to an epimer pair in the chondroitin sulfate glycoform class does not provide the same result. A series of experiments have been conducted in which glycosam...

When compared to other complex carbohydrate polymers. heparin and heparan sulfate present unique challenges for structural characterization. Special finesse must be used to determine their monoor disaccharide compositions, their sulfate contents, and their disaccharide sequences Approaches that have been developed for addressing these difficult problems include selective enzyme cleavage, select...