When the concentration dependence of an enzymic reaction or a transport process can be described by the sum of two Michaelis-Menten terms, reliable data that relate reaction rate and substrate concentration can be obtained even when as much as 70 per cent of substrate was consumed during the assay. Each data pair consists of the average reaction rate during an assay and the concentration where ...

Neutron reflectometry was used to determine the distribution of salt ions and water in thin poly(acrylic acid) and poly(allylamine hydrochloride) polyelectrolyte multilayers assembled with and without salt. Increasing salt concentration reverses the exclusion of water from the substrate region, eventually leading to an asymmetric segregation of water near the substrate at high salt concentratio...

In this contribution, the Co doped SnO2 transparent semi-conducting films are prepared by spray pyrolysis technique and the influence of N2-and Ar-ambient annealing on their structural, electrical and optical properties are studied. The SnO2:Co thin films were deposited on the glass substrate at substrate temperature of 480 ˚C using an aqueous-ethanol solution consisting of tin and cobalt chlor...

1. A theoretical appraisal of the alternative pathway mechanism for a two-substrate enzyme shows that this mechanism is capable of giving rise to apparent substrate inhibition or substrate activation (Dalziel, 1958). It has now been shown that these phenomena may occur simultaneously in the following ways. With certain relationships between the kinetic parameters and the constant concentration ...

Rate studies using phosphoglycerate kinase (PGK)--glyceraldehyde-3-phosphate dehydrogenase (GPDH) enzyme pair have been carried out to distinguish between the two mechanisms of intermediate metabolite transfer, namely diffusion through the solvent versus "substrate channelling" within an enzyme-enzyme complex. A procedure has been described for the assay of the rates of PGK-catalysed and the PG...

Effect of substrate temperatures, spray rates, solution volumes, and nickel concentration on structural optical properties NiO thin films prepared by pyrolysis

In  this paper  thin  films of  tin sulfide (SnS) were deposited on  the glass substrates using spray pyrolysis method with the substrate temperatures in the range of 400–600℃, keeping the other deposition parameters constant. In  this work  the characteristic of SnS  thin  films  investigated. The XRD pattern and optical transmittance of thin films also are discussed. With the change in concen...

Studies on Simultaneous Saccharification and Fermentation (SSF) of corn flour, a major agricultural product as the substrate using starch digesting glucoamylase enzyme derived from Aspergillus niger and non starch digesting and sugar fermenting Saccharomyces cerevisiae in a batch fermentation. Experiments based on Central Composite Design (CCD) were conducted to study the effect of substrate co...

Studies on Simultaneous Saccharification and Fermentation (SSF) of corn flour, a major agricultural product as the substrate using starch digesting glucoamylase enzyme derived from Aspergillus niger and non starch digesting and sugar fermenting Saccharomyces cerevisiae in a batch fermentation. Experiments based on Central Composite Design (CCD) were conducted to study the effect of substrate co...