The Escherichia coli dam adenine-N6 methyltransferase modifies DNA at GATC sequences. It is involved in post-replicative mismatch repair, control of DNA replication and gene regulation. We show that E. coli dam acts as a functional monomer and methylates only one strand of the DNA in each binding event. The preferred way of ternary complex assembly is that the enzyme first binds to DNA and then...

We reported previously that a Salmonella enterica serovar Enteritidis dam mutant expressing a truncated Dam protein does not agglutinate in the presence of specific antibodies against O9 polysaccharide. Here we investigate the participation of Dam in lipopolysaccharide (LPS) synthesis in Salmonella. The LPS O-antigen profiles of a dam null mutant (SEDeltadam) and the Salmonella serovar Enteriti...

Air-water flow experiments were conducted on some flat and pooled stepped spillways with slopes of 8.9° and 26.6° in transition and skimming flows. The study comprised the observations of the flow patterns, characteristic air-water flow properties, and energy dissipation performances. The air-water flow properties showed some differences in terms of interfacial velocity, bubble count rate, and ...

The classical dam break problem has become the de facto standard in validating the nonlinear shallow water equations solvers. Moreover, the Nonlinear Shallow Water Equations (NSWE) are widely used for flooding simulations. While applied mathematics community is essentially focused on developing new numerical schemes, we tried to examine the validity of the mathematical model under consideration...

The Burdekin River, Queensland, Australia drains a catchment area of ~130,000 km and contributes approximately 30% of the total sediment supplied (~ 3.8 t) to the Great Barrier Reef Lagoon from all Great Barrier Reef catchments. Much of this watershed (~115,000 km) drains into the Burdekin Falls Dam, the largest dam reservoir in Queensland (1,860 GL capacity; 224 km surface area; average depth ...

Most dentists are educated in rubber dam use in dental school, but there is often disparity between what is taught for various restorative procedures and what is practiced in the private sector. It is a common, although undocumented, belief that few practicing dentists routinely use rubber dam isolation. This study repeated a survey conducted in 1985 evaluating U.S. general dentists' attitudes ...

BACKGROUND This survey study aimed at investigating the frequency of rubber dam use during root canal treatment, identifying influencing factors for not using it by Saudi general dental practitioners (GDPs) and endodontists. It also aimed at identifying measures that increase rubber dam usage. METHODS After obtaining an ethical approval, two pilot studies were conducted on staff members at Ta...

Dam methylation is an essential factor involved in the virulence of an increasing number of bacterial pathogens including Salmonella enterica. Lack of Dam methylation causes severe attenuation in animal models. It has been proposed that dysregulation of Dam activity is potentially a general strategy for the generation of vaccines against bacterial pathogens. In this review, we focus our attenti...

Multiple regression of genetic evaluations for milk yields of first lactations of 159 Holstein bulls on sire, dam, and maternal grandsire evaluations indicates that the weight for estimated transmitting ability of the dam calculated from the first record of the dam and first records of her herdmates is about as theoretically expected and is markedly superior both to estimated transmitting abili...