Pathological diagnosis relies on the visual inspection of histologically stained thin tissue specimens, where different types stains are applied to bring contrast and highlight various desired histological features. However, destructive histochemical staining procedures usually irreversible, making it very difficult obtain multiple same section. Here, we demonstrate a virtual stain transfer fra...

Pathology is practiced by visual inspection of histochemically stained slides. Most commonly, the hematoxylin and eosin (H&E) stain used in diagnostic workflow it gold standard for cancer diagnosis. However, many cases, especially non-neoplastic diseases, additional "special stains" are to provide different levels contrast color tissue components allow pathologists get a clearer picture. In thi...

a three day old neonate was referred to our department with a problem of a sternal cleft. sternal clefts are often associated with a myriad of other abnormalities ranging from mild to severe. we present our experience with such a problem, and review the current literature concerning it.

To investigate the direct effect of leukocyte adherence to microvessel walls on microvessel permeability, we developed a method to measure changes in hydraulic conductivity (L(p)) before and after leukocyte adhesion in individually perfused venular microvessels in frog mesentery. In 19 microvessels that were initially free of leukocyte sticking or rolling along the vessel wall, control L(p) was...

From the time the Leishman stain has been used for staining blood cells and parasites, it has been dissolved in acetone-free good quality methyl alcohol. Dissolving the stain in inferiorquality methyl alcohol or ethyl alcohol always gives poor results and hsematologists and parasitologists requiring precision work would always use acetone-free methyl alcohol of the best quality. After the secon...

Malassezia spp. are lipid-dependent yeasts that have been related to skin mycobiota and dermatological systemic diseases. Study of lipid droplets (LDs) is relevant elucidate the unknown role these organelles in gain a broader overview metabolism Malassezia. Here, we standardized two protocols for analysis LDs M. pachydermatis globosa. The first describes co-staining confocal laser-scanning fluo...