BACKGROUND The purpose of the study was to evaluate the long-term clinical tracking of magnetically labeled stem cells after intracerebroventricular transplantation as well as to investigate in vitro feasibility for magnetic guidance of cell therapy within large fluid compartments. METHOD After approval by our Institutional Review Board, an 18-month-old patient, diagnosed as being in a vegeta...

PURPOSE Magnetic resonance imaging (MRI) using contrast agents like superparamagnetic iron oxide (SPIO) is an extremely versatile technique to diagnose diseases and to monitor treatment. This study tested the relative importance of particle size and surface coating for the optimization of MRI contrast and labeling efficiency of macrophages migrating to remote inflammation sites. MATERIALS AND...

BACKGROUND Cell tracking is a powerful tool to understand cellular migration, dynamics, homing and function of stem cell transplants. Nanoparticles represent possible stem cell tracers, but they differ in cellular uptake and side effects. Their properties can be modified by coating with different biocompatible polymers. To test if a coating polymer, poly(L-lysine), can improve the biocompatibil...

The prevalence of cancer has seen significant growth over the last three decades, with the American Cancer Society now reporting that one in every three Americans will be afflicted with some form of cancer in their lifetime. The debilitating effects of cancer permeate beyond obvious biological ramifications, often adversely affecting patients on emotional and financial levels as well. While sig...

The relaxation rates of iron-oxide nanoparticles compartmentalized within cells were studied and found to satisfy predictions of the static dephasing (SD) regime theory. THP-1 cells in cell culture were loaded using two different iron-oxide nanoparticles (superparamagnetic iron-oxide (SPIO) and ultrasmall SPIO (USPIO)) with four different iron concentrations (0.05, 0.1, 0.2, and 0.3 mg/ml) and ...

'Transfection Agents (TA) mixed with FDA approved superparamagnetic iron oxide nanoparticle (SPIO), effectively coating the SPIO, resulted in endosomal incorporation of iron. (Stem) Cells were incubated with TASPIO mixtures and evaluated. Intracytoplasmic particles stained with Prussian Blue were detected and MR images and relaxometry of labeled cells revealed decrease in signal intensity on T2...