) Cancer stem cells (CSCs) are believed responsible for the tumor initiation and the resistance to current cancer therapies such as chemoand radiotherapy, which also lead to tumor recurrences and metastasis. By using a 3-D sphere culture method that favors the growth of self-renewal cells, we have successfully isolated sphere forming cells (SFCs) from cervical cancer HeLa and SiHa cells. It is ...

The importance of 3-dimensional (3D) topography in influencing neural stem and progenitor cell (NPC) phenotype is widely acknowledged yet challenging to study. When dissociated from embryonic or post-natal brain, single NPCs will proliferate in suspension to form neurospheres. Daughter cells within these cultures spontaneously adopt distinct developmental lineages (neurons, oligodendrocytes, an...

Malignant pleural mesothelioma (MPM) is an aggressive cancer. MPM cells express aquaporin-1 (AQP1) that in other cancers has been shown to participate in the tumor metastasis processes. However, in MPM patients AQP1 overexpression is an independent prognostic factor favoring survival. In this study we aimed at evaluating the role of AQP1 in cell adhesion, migration, and tumor sphere formation i...

Epimorphin was recently described as a mesenchymal factor modulating morphogenesis of murine mammary ducts, skin, liver, and lung in vitro. In this study epimorphin was analyzed in a human, pancreatic adenocarcinoma cell line (A818-6) which develops single layer epithelial hollow spheres resembling normal pancreatic ductal structures in vitro. Soluble 34- and 31-kD isoforms of epimorphin were f...

The spiral ganglion is an essential functional component of the peripheral auditory system. Most types of hearing loss are associated with spiral ganglion cell degeneration which is irreversible due to the inner ear's lack of regenerative capacity. Recent studies revealed the existence of stem cells in the postnatal spiral ganglion, which gives rise to the hope that these cells might be useful ...

Intradermal adipose tissue plays an essential role for hair follicles (HFs) regeneration by regulating hair cycles. However, the effect of reconstruction of HFs and the involvement of adipose-related cells are poorly understood. We investigated assembly strategies for the interactions of dermal papilla (DP) cells with adipose-derived stem cells (ASCs) in promoting hair formation. DP cells lose ...

The tumor-initiating capacity of primary human breast cancer cells is maintained in vitro by culturing these cells as spheres/aggregates. Inoculation of small cell numbers derived from these non-adherent cultures leads to rapid xenograft tumor formation in mice. Accordingly, injection of more differentiated monolayer cells derived from spheres results in significantly decelerated tumor growth. ...

This essay about the phenomenon of human self-overlapping presents an evident plurality of approaches and theoretical definitions of this phenomenon. On the basis of historical, philosophical, psychological and sociological starting points the author tries to systemise knowledge and creates his own, temporary dual structure of the selfoverlapping phenomenon. The given phenomenon is suitable for...

Drawing on a case study of public relations in the UK charity sector, this article argues that cultural intermediary research urgently requires more sustained focus politics and political understood as power relations, party projects such marketization neoliberalism. While wide-ranging has analysed how intermediaries mediate relationship between culture economy, been at expense an in-depth anal...

The collective monograph “The media sphere as space for the development of legal culture in modern Russia” dedicated to study educational potential and human rights Russia is reviewed. thematical content book, its structure are considered, attention paid relevance research results obtained by scientists. author’s interpretation factors influence new communications technologies on an individual ...