To establish an effective and concise procedure for determining the concentrations of spermidine and spermine, several functional molecules based on phenolphthalein and two crown loops were constructed. Host 5 with a dimethylamino group showed excellent selectivity for spermidine and spermine among other biogenic amines and high sensitivity compared with basic host 1.

For bone tissue engineering, it is important that mesenchymal stem cells (MSCs) differentiate into osteoblasts. To develop a method for differentiation of adipose tissue-derived mesenchymal stem cells (AT-MSCs) along the osteogenic lineage, we studied the effect of polyamines, which are organic cations implicated in bone growth and development, on differentiation of AT-MSCs. Treatment of goat-d...

Studies are presented on the influence of polyamines on prostatic chromatin- and non-histone-protein-associated protein kinase reactions involving both exogenous and endogenous substrates. The activities toward the model acidic protein substrate, dephosphophosvitin, were maximal at 160--200mM-NaCl (or -KCl or -NH4Cl). Under these conditions, spermidine and spermine added in concentrations up to...

Putrescine was first isolated from putrifying meat and was thought of as a decomposition product; spermine was named from its occurrence in semen. These polyamines, however, are now known to have important roles in cell growth and differentiation. Their physiological significance can be studied by analyzing the consequences of depletion of the cellular polyamine content. The results include arr...

A vast number of studies have linked polyamines to numerous cellular functions but less is known about the possible physiological functions of the individual polyamines. The specific gene targeting technique has provided a new potential way to resolve this mystery. We generated two separate embryonic stem (ES) cell lines where either spermine synthase gene (SPMSy) or spermidine/spermine N-acety...

The Cu(I)-catalyzed N,N'-diarylation of natural diamines and polyamines such as putrescine, cadaverine, spermine, spermidine and their homologues is described. Aryl iodides bearing electron-donating and electron-withdrawing groups have been employed in the study. The CuI/2-(isobutyryl)cyclohexanone/DMF catalytic system has found to be more efficient in the diarylation of diamines and spermine w...

In the present study, the spermine (SPM) analogue N1N12-bis(ethyl)spermine (BESPM) is compared with SPM in its ability to regulate ornithine decarboxylase (ODC) and S-adenosyl-L-methionine decarboxylase (AdoMetDC) activities in intact L1210 cells and in the mechanism(s) by which this is accomplished. Unlike the comparable spermidine (SPD) analogue N1N8-bis(ethyl)spermidine, which regulates only...

We have analyzed the function of spermine in the aminoacylation of tRNA Val by the valyl-tRNA synthetase of Escherichia coli. Our results indicate that Mg2+ is required for the aminoacylation reaction as well as for the ATP-PPi exchange catalyzed by this enzyme. The apparent stimulation by spermine is a function of the tRNA used, which appears to contain bound cations even after dialysis agains...