The present study was undertaken to clarify changes in secretions of FSH, LH, inhibin and testosterone, and sperm motility after bilateral vasectomy in adult male rats. Bilateral vasectomy was created surgically (treated group) and intact rats were used as control (control group). On days 3, 5, 7, 14, 30, 60, and 90 after surgery, plasma concentrations of FSH, LH, inhibin, and testosterone were...

Polycyclic aromatic hydrocarbons (PAHs), like benzo[a]pyrene (BaP), are ubiquitous environmental pollutants formed by the incomplete combustion of organic materials. The tripeptide glutathione (GSH) is a major antioxidant and is important in detoxification of PAH metabolites. Mice null for the modifier subunit of glutamate cysteine ligase (Gclm), the rate-limiting enzyme in GSH synthesis, have ...

The shape of the flagellar beat determines the path along which a sperm cell swims. If the flagellum bends periodically about a curved mean shape then the sperm will follow a path with non-zero curvature. To test a simple hydrodynamic theory of flagellar propulsion known as resistive force theory, we conducted high-precision measurements of the head and flagellum motions during circular swimmin...

Doxorubicin is a potent antitumor agent used against many cancers. It has genotoxic effect on human non-tumor (normal) cells. Hence, it is very important to reduce its toxicity to the normal cells. This goal can be achieved by concurrent administration of free radical scavenging agents, like antioxidants. In the present study, adult male albino rats of Wistar strain are administered with Doxoru...

The mammalian Y chromosome is essential for spermatogenesis, which is characterized by sperm cell differentiation and chromatin condensation for acquisition of correct shape of the sperm. Deletions of the male-specific region of the mouse Y chromosome long arm (MSYq), harboring multiple copies of a few genes, lead to sperm head defects and impaired fertility. Using chromatin immunoprecipitation...

The conventional assessment of human semen, especially sperm movement characteristics, is a highly subjective assessment, with considerable intra- and inter-technician variability. Computer-assisted sperm analysis (CASA) systems provide a rapid and automated assessment of the parameters of sperm motion, together with improved standardization and quality control. However, it should be noted that...

BACKGROUND Cellular and molecular mechanisms leading to elongated sperm heads are not known. We have analysed the nuclear status of spermatozoa with elongated heads. METHODS Fourteen men with at least 30% of spermatozoa with an elongated nucleus were studied and compared with five fertile men as controls. Sperm morphology was analysed by a quantitative ultrastructural analysis. Sperm chromoso...

The effects of colchicine on spermiogenesis in the mouse were examined. Mice were injected intratesticularly with colchicine in concentrations of 10(-4) M to 10(-6) M for various periods of time. The ultrastructure of the testes was examined and abnormal sperm in the epididymis were scored. Colchicine degraded microtubules in the testis and the treatment caused abnormalities of the head and acr...

Mammalian sperm cells are activated prior to fertilization by high bicarbonate levels, which facilitate lipoprotein-mediated cholesterol efflux. The role of bicarbonate and cholesterol acceptors on the cholesterol organization in the sperm plasma membrane was tested. Bicarbonate induced an albumin-independent change in lipid architecture that was detectable by an increase in merocyanine stainin...

The axolotl has extraordinary regeneration capacity compared to other vertebrates. This remarkable potential been attributed its life-long neoteny, characterized by the exhibition of embryonic characteristics at adult stage. A recent study provided a detailed morphological analysis sperm morphology Ambystoma mexicanum using routine and histological techniques. primary purpose present is describ...