Most free-living barnacles are hermaphroditic, and eggs are presumed to be fertilized either by pseudo-copulation or self-fertilization. Although the common northeast Pacific intertidal gooseneck barnacle, Pollicipes polymerus, is believed only to cross-fertilize, some isolated individuals well outside penis range nonetheless bear fertilized eggs. They must therefore either self-fertilize or-co...

Active immune mechanism in the female reproductive tract may produce high levels of anti-seminal/sperm antibodies. Generated antibodies in the process of isoimmunization seem to be associated with female immune infertility. The aim of our study consists in the profiling of specific serum immunoglobulin classes and subclasses in infertile women. We focus on the distribution of serum seminal/sper...

Excess reactive oxygen species (ROS) beyond the scavenging capacity of antioxidants leads to DNA damage and oxidation of lipoprotein components at the cellular and subcellular level. The oxidative stress (OS) adversely affects sperm function by altering membrane fluidity, permeability and impairs sperm functional competence. In the present study, the OS status in seminal plasma and blood serum ...

The separation of mammalian spermatozoa from seminal plasma is a practice routinely used in assisted reproductive technology applications and in sperm biomedical studies. The sperm isolation method is critically important for clinicians preparing sperm samples to be used in reproductive biotechnologies, veterinary medicine and animal husbandry. This practice has been used for several years in t...

Unexplained infertility requires that more sensitive and mechanism-based biomarkers should be developed and used independently of or in addition to conventional semen parameters for an infertility diagnosis. In the present study, semen samples were collected from young men participating in the Male Reproductive Health in Chongqing College students (MARCHS) cohort study in the follow-up stage in...

Objectives: This study aimed to evaluate the effect of anthocyanin on oxidative stress, sperm, and testis structure in diabetic rats induced by streptozotocin (STZ). Materials Methods: In this experimental research, diabetes was a single intraperitoneal injection STZ (50 mg/ kg). A total 64 were assigned into four groups as follows: control group, group daily administrated with at dose 100 mg/k...

Determining causal relationships between distinct chromatin features and gene expression, and ultimately cell behavior, remains a major challenge. Recent developments in targetable epigenome-editing tools enable us to assign direct transcriptional and functional consequences to locus-specific chromatin modifications. This Protocol Review discusses the unprecedented opportunity that CRISPR/Cas9 ...

Introduction Sperm interacts with the female reproductive tract and oocyte through proteins, these cell-to-cell interactions may play a role in sperm fertility. For consideration of protein as potential marker fertility, there must be variability expressed among animals. The proteins dystroglycan (DAG1) plasma serine protease inhibitor (SERPINA5) have been reported to interactions. Thus, object...

BACKGROUND Body size, nutrition and socioeconomic position (SEP) in early life have been associated with a range of later life health outcomes. Epigenetic regulation is one mechanism through which these early life factors may impact later life health. The aim of this review protocol is to outline procedures to document the influence of body size, nutrition and SEP in early life on the epigenome...

DNA methylation is the current strategy in the field of biomarker discovery due to its prognostic efficiency. Its role in prognosis and early diagnosis has been recognized in various types of cancer. Sepsis still remains one of the major causes of neonatal mortality. Delay in diagnosis of sepsis leads to treatment difficulties and poor outcome. In this study, we have done an epigenome wide sear...